THE YEAST MUD2 PROTEIN - AN INTERACTION WITH PRP11 DEFINES A BRIDGE BETWEEN COMMITMENT COMPLEXES AND U2 SNRNP ADDITION
THE YEAST MUD2 PROTEIN - AN INTERACTION WITH PRP11 DEFINES A BRIDGE BETWEEN COMMITMENT COMPLEXES AND U2 SNRNP ADDITION
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DOI：
10.1101/gad.8.7.843
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发表时间：
1994-04-01
影响因子：
10.5
通讯作者：
ROSBASH, M
中科院分区：
文献类型：
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作者：
ABOVICH, N;LIAO, XLC;ROSBASH, M
在表征一系列酵母（酿酒酵母）突变体的合成致死与U1 RNA，我们已经确定了一个酵母基因（MUD 2）的序列相似性，充分研究后生动物剪接因子U2 AF 65。生物化学表征表明MUD 2基因产物（MUD 2 P）直接接触前mRNA，并且是在酵母提取物中的早期剪接体组装期间形成的前mRNA-U1 snRNP复合物（定型复合物）的组分。与U1 snRNP本身不同，MUD 2 P与前mRNA的结合依赖于适当的酵母分支点序列。遗传实验表明MUD 2 P影响U2 snRNP的添加。此外，在双杂交系统中的实验表明，PRP 11 P，最近确定的U2 snRNP的组成部分，可以直接与MUD 2 P相互作用。实验鉴定了在剪接体组装期间发生的特异性snRNP间蛋白质-蛋白质接触，并且更普遍地支持U2 AF 65和MUD 2 P之间的实质性功能相似性。
In characterizing a series of yeast (Saccharomyces cerevisiae) mutants synthetic lethal with U1 RNA, we have identified a yeast gene (MUD2) with sequence similarity to the well-studied metazoan splicing factor U2AF65. The biochemical characterization indicates that the MUD2 gene product (MUD2P) contacts pre-mRNA directly and is a component of the pre-mRNA-U1 snRNP complex (commitment complex) that forms during early spliceosome assembly in yeast extracts. Unlike U1 snRNP itself, the association of MUD2P with pre-mRNA is dependent on a proper yeast branchpoint sequence. Genetic experiments indicate that MUD2P affects U2 snRNP addition. Moreover, experiments in the two-hybrid system show that PRP11P, a recently identified component of U2 snRNP, can interact directly with MUD2P. The experiments identify a specific inter-snRNP protein-protein contact that occurs during spliceosome assembly and more generally support substantial functional similarity between U2AF65 and MUD2P.