A comparison of two common bile duct ligation methods to establish hepatopulmonary syndrome animal models
A comparison of two common bile duct ligation methods to establish hepatopulmonary syndrome animal models
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两种胆总管结扎方法建立肝肺综合征动物模型的比较
DOI：
10.1177/0023677214558701
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发表时间：
2015-01-01
影响因子：
2.4
通讯作者：
Lu, K.
中科院分区：
文献类型：
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作者：
Yang, Y.;Chen, B.;Lu, K.
目前胆总管结扎(CBDL)诱导的肝肺综合征(HPS)动物模型的主要缺点是死亡率极高，阻碍了实验研究。本研究的目的是探索一种改进的CBDL方法，目的是建立一种简单、可重复性的单次CBDL治疗大鼠HPS模型。雄性SD大鼠分为两组：(1)上胆总管结扎组(UCBDL组，n=40)，第一次结扎在肝管交界处附近，第二次结扎在胰管入口上方；(2)胆总管中段结扎(MCBDL)组(n=40)，第一次结扎在胆总管中部，第二次结扎在胰管入口上方。通过脉搏血氧饱和度、动脉血分析、组织病理学和脑内~(99m)Tm标记白蛋白大聚集体摄取(反映肺内血管扩张)对CBDL诱导的HPS大鼠进行评估。UCBDL组和MCBDL组的死亡率分别为42.5%和77.5%(P
The major drawback of the current common bile duct ligation (CBDL)-induced hepatopulmonary syndrome (HPS) animal model is the extremely high mortality rate that hinders experimental studies. The purpose of this study was to investigate an improved method of CBDL with the goal of developing a simple and reproducible rat HPS model after a single CBDL treatment. Two groups of male Sprague-Dawley rats underwent separate methods of CBDL: (1) the upper common bile duct ligation (UCBDL) group (n=40), in which the first ligature was made near the junction of the hepatic ducts, and the second ligature was made above the entrance of the pancreatic duct; (2) the middle of the common bile duct ligation (MCBDL) group (n=40), in which the first ligature was made in the middle of the common bile duct, and the second ligature was made above the entrance of the pancreatic duct. The CBDL-induced HPS rats were evaluated by pulse oximeter, arterial blood analysis, histopathology, and cerebral uptake of intravenous technetium-99m-labeled albumin macroaggregates (which reflects intrapulmonary vascular dilation). The mortality rates of the UCBDL group and the MCBDL group were 42.5% and 77.5%, respectively (P