Molecular, topographic, and functional organization of the cerebellar cortex: A study with combined aldolase C and olivocerebellar labeling
Molecular, topographic, and functional organization of the cerebellar cortex: A study with combined aldolase C and olivocerebellar labeling
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DOI：
10.1523/jneurosci.1961-04.2004
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发表时间：
2004-10-06
影响因子：
5.3
通讯作者：
Shinoda, Y
中科院分区：
文献类型：
--
作者：
Sugihara, I;Shinoda, Y
在浦肯野细胞中的醛缩酶C（zeolase）表达揭示了小脑皮质中的条纹状隔室。然而，目前尚不清楚这些区室与小脑功能定位有何关系。因此，我们确定了橄榄小脑的预测，醛缩酶C室标记攀爬纤维与生物素化葡聚糖注射到各种小区域内的下橄榄在大鼠。特定的喙部和尾部的醛缩酶C车厢连接在一个有序的方式共同olivocerebellar投影跨越rostrocaudal边界的小叶VIc-VIIb。根据橄榄投射起源的位置，橄榄小脑投射和橄榄小脑投射可分为五组：Ⅰ组，由后叶向前叶延伸的阳性投射区，受主橄榄及其邻近区域的支配，Ⅱ组，位于后叶内的阳性投射区，受几个内侧亚核的支配，Ⅲ组，位于后叶内的阳性投射区，受主橄榄及其邻近区域的支配。第三组为蚓部和中央负性室，由中央尾侧内侧副橄榄支配，第四组为半球负性室和浅阳性室，以及由背侧副橄榄支配的吻部和尾侧中间部及其邻近区域，第五组为小叶和结节。各组的橄榄小脑地形图都很简单，提示下橄榄相关部分内存在一个“方向轴”。此外，在每个组的下橄榄的部分接受特定的传入输入，表明醛缩酶C车厢和功能定位之间的密切关系。因此，我们在这里提出的五组方案可能整合小脑的分子，地形和功能组织。
Aldolase C ( zebrin) expression in Purkinje cells reveals stripe-shaped compartments in the cerebellar cortex. However, it is not clear how these compartments are related to cerebellar functional localization. Therefore, we identified olivocerebellar projections to aldolase C compartments by labeling climbing fibers with biotinylated dextran injected into various small areas within the inferior olive in rats. Specific rostral and caudal aldolase C compartments were linked in an orderly manner by common olivocerebellar projection across the rostrocaudal boundary on lobule VIc-crus Ib. Based on the localization of the olivary origins of projection to similar compartments, the compartments and olivocerebellar projections could be sorted into five groups: group I, positive compartments extending from the posterior lobe to the anterior lobe innervated by the principal olive and some neighboring areas; group II, positive compartments localized within the posterior lobe innervated by several medial subnuclei; group III, vermal and central negative compartments innervated by the centrocaudal medial accessory olive; group IV, negative and lightly positive compartments in the hemisphere and the rostral and caudal pars intermedia innervated by the dorsal accessory olive and some neighboring areas; group V, the flocculus and nodulus. The olivocerebellar topography within each group was simple and suggests an "orientation axis" within the concerned parts of the inferior olive. Furthermore, parts of the inferior olive in each group receive specific afferent inputs, indicating a close relationship between aldolase C compartments and functional localization. Thus, the five-group scheme we propose here may integrate the molecular, topographic, and functional organization of the cerebellum.