The pesticides endosulfan, toxaphene, and dieldrin have estrogenic effects on human estrogen-sensitive cells.
The pesticides endosulfan, toxaphene, and dieldrin have estrogenic effects on human estrogen-sensitive cells.
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DOI：
10.1289/ehp.94102380
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发表时间：
1994-04
影响因子：
10.4
通讯作者：
Sonnenschein C
中科院分区：
文献类型：
--
作者：
Soto AM;Chung KL;Sonnenschein C
滴滴涕和十氯酮等雌激素农药会对生殖产生有害影响。使用“培养物”生物测定来评估几种农药的雌激素性。 E-筛选测试使用人乳腺雌激素敏感的 MCF7 细胞，并比较在存在（阳性对照）或不存在（阴性对照）雌二醇以及怀疑具有雌激素作用的不同浓度的异生物质的情况下，在补充有 5% 木炭-葡聚糖剥离的人血清的培养基中培养 6 天后获得的细胞产量。在测试的有机氯农药中，毒杀芬、狄氏剂和硫丹的雌激素特性与滴滴涕和十氯酮相当；已知后者在啮齿动物模型中具有雌激素作用。电子筛查测试还显示，雌激素化学物质可能会累积作用；当混合在一起时，它们以低于单独施用每种化合物所需的浓度诱导雌激素反应。
Estrogenic pesticides such as DDT and chlordecone generate deleterious reproductive effects. An "in culture" bioassay was used to assess the estrogenicity of several pesticides. The E-screen test uses human breast estrogen-sensitive MCF7 cells and compares the cell yield achieved after 6 days of culture in medium supplemented with 5% charcoal-dextran stripped human serum in the presence (positive control) or absence (negative control) of estradiol and with diverse concentrations of xenobiotics suspected of being estrogenic. Among the organochlorine pesticides tested, toxaphene, dieldrin, and endosulfan had estrogenic properties comparable to those of DDT and chlordecone; the latter are known to be estrogenic in rodent models. The E-screen test also revealed that estrogenic chemicals may act cumulatively; when mixed together they induce estrogenic responses at concentrations lower than those required when each compound is administered alone.