Evaluación de la citotoxicidad de la aflatoxina y la fumonisina en células intestinales de porcino
Evaluación de la citotoxicidad de la aflatoxina y la fumonisina en células intestinales de porcino
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DOI：
10.1016/s1130-1406(07)70029-3
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发表时间：
2007-06
影响因子：
1.9
通讯作者：
Juan Carlos Angulo García;C. M. Ramos;P. Pinton;S. Elvira;I. Oswald
中科院分区：
文献类型：
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作者：
Juan Carlos Angulo García;C. M. Ramos;P. Pinton;S. Elvira;I. Oswald
黄曲霉毒素（AFB）和伏马菌素（FB）的共存已在世界许多地方被广泛报道。然而，很少有研究描述两种真菌毒素在体内和/或体外的协同作用。本研究旨在探讨黄曲霉毒素（AFB）和纤维蛋白原（FB）对猪小肠上皮细胞（IPEC-1）形态、细胞增殖能力、细胞毒性和白细胞介素-8（IL-8）合成的影响。在细胞形态方面，只有在AFB（50 μ M）和FB（500 μ M）浓度较高时才受到影响。然而，当AFB/FB（分别为1.3/3.7、2/3.7和5/10 μ M）组合存在时，细胞增殖、细胞损伤和IL-8的合成受到影响，这与类似浓度的这些真菌毒素的单独作用所显示的结果（p< 0.05）。我们的数据表明，低浓度的AFB/FB组合显示出协同效应，改变了细胞形态生理学，这可能意味着在体内其他毒素或生物制剂的进入，改变了肠道屏障，对人类或动物健康产生负面影响。
The coexistence of the aflatoxin (AFB) and fumonisin (FB) has been widely documented in many parts of the world. However, few studies describing the synergy effect of both mycotoxins in vivo and/or in vitro are available. The objective of this study consisted on evaluating the effect of AFB and FB on the morphology, the capacity of cellular proliferation, cytotoxicity and interleukin-8 (IL-8) synthesis in a porcine intestinal epithelial cell line (IPEC-1). Concerning to the cellular morphology it was only affected in the concentrations higher of AFB (50 microM) and FB (500 microM). However, the cellular proliferation, the cellular damage and synthesis of IL-8 they were affected when present in combination the AFB/FB (1.3/3.7; 2/3.7 and 5/10 microM respectively) with that showed by the individual effect of similar concentrations of these mycotoxins (p< 0.05). Our data indicate that the combination of AFB/FB in low concentrations showed a synergy effect, altering the cellular morphophysiology, which can imply in vivo the entrance of other toxins or biological agents for alteration of the intestinal barrier impacting negatively in the human or animal health.