Characterization of virus DNA synthesized in KB cells infected with two temperature-sensitive mutants of adenovirus type 5.
Characterization of virus DNA synthesized in KB cells infected with two temperature-sensitive mutants of adenovirus type 5.
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感染 5 型腺病毒两种温度敏感突变体的 KB 细胞中合成的病毒 DNA 的表征。
DOI：
10.1099/0022-1317-44-1-25
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
M. van Maarschalkerweerd
中科院分区：
文献类型：
--
作者：
J. Sussenbach;M. Kuijk;M. van Maarschalkerweerd
KB 细胞被 H5ts36 或 H5ts125 感染，这两种腺病毒 5 型 (Ad5) 突变体具有温度敏感的病毒 DNA 合成。感染在不允许的温度下和注射后 16 小时开始。温度降低至允许温度。下调后不久，H5ts125 感染的细胞显示出病毒来源的纯单链 DNA 的积累，而在 H5ts36 感染的细胞中未观察到这种情况。该单链 DNA 已通过杂交和沉降分析进行了表征。结果发现，单链DNA源自两条互补链，并且由短片段组成。在DNA结合蛋白量减少的条件下，单链DNA在H5ts125感染的细胞中积累，这表明DNA结合蛋白不仅参与起始，而且参与新生链的延伸。
KB cells were infected with H5ts36 or H5ts125, two adenovirus type 5 (Ad5) mutants with a temperature-sensitive synthesis of virus DNA. Infection was started at the nonpermissive temperature and at 16 h p.i. the temperature was shifted down to the permissive temperature. Shortly after the shift-down H5ts125-infected cells showed an accumulation of purely single-stranded DNA of virus origin, which was not observed in H5ts36-infected cells. This single-stranded DNA has been characterized by hybridization and sedimentation analysis. It was found that the single-stranded DNA was derived from both complementary strands and consisted of short fragments. The observation that the single-stranded DNA accumulates in H5ts125-infected cells under conditions in which the amount of DNA binding protein is reduced, suggests that the DNA-binding protein is not only involved in initiation, but also in elongation of nascent strands.