Noncentrosomal microtubules regulate autophagosome transport through CAMSAP2-EB1 cross-talk
Noncentrosomal microtubules regulate autophagosome transport through CAMSAP2-EB1 cross-talk
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非中心体微管通过 CAMSAP2-EB1 串扰调节自噬体运输
DOI：
10.1002/1873-3468.12758
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Meng W.
中科院分区：
文献类型：
--
作者：
Wei J.;Xu H.;Meng W.
自噬是维持细胞内稳态的重要降解途径，微管在自噬的许多步骤中发挥着重要作用。在许多细胞中，MT网络由中心体MT和非中心体MT组成。然而，目前尚不清楚非中心体MT及其结合蛋白是否参与自噬。在这里，我们在HeLa细胞中发现，钙调蛋白调节血影蛋白相关蛋白2（CAMSAP 2），一种非中心体MT负末端稳定蛋白，通过MT动力学调节自噬体的逆行转运。CAMSAP2与EB1合作调节MT+末端的末端结合蛋白1（EB1）行为，MT生长方向和自噬体转运。CAMSAP 2和EB 1在胞质溶胶之间的关联可以调节EB 1与MT+末端的结合。总的来说，我们的数据表明，非中心体MT通过CAMSAP 2和EB 1之间的相互作用调节自噬。
Microtubules (MTs) play essential roles in many steps of autophagy, an important degradation pathway in the maintenance of cellular homoeostasis. In many cells, MT networks are comprised of centrosomal MTs and noncentrosomal MTs. However, it is unknown whether noncentrosomal MTs and its binding proteins are involved in autophagy. Here, we show in HeLa cells that calmodulin‐regulated spectrin‐associated protein 2 (CAMSAP2), a noncentrosomal MT minus‐end stabilizing protein, regulates retrograde transport of autophagosomes through MT dynamics. CAMSAP2 cooperates with EB1 to regulate end‐binding protein 1 (EB1) behaviour at MT plus ends, MT growth directions and autophagosome transport. An association between CAMSAP2 and EB1 in the cytosol may modulate EB1 binding to MT plus ends. Collectively, our data indicate that noncentrosomal MTs regulate autophagy through a cross‐talk between CAMSAP2 and EB1.