Effects of dissolved organic matter derived from cow manure on heavy metal(loid)s and bacterial community dynamics in mercury-thallium mining waste slag
Effects of dissolved organic matter derived from cow manure on heavy metal(loid)s and bacterial community dynamics in mercury-thallium mining waste slag
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DOI：
10.1007/s10653-023-01607-7
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发表时间：
2023-05
影响因子：
4.2
通讯作者：
Yu-Fen He;Youfa Luo;C. Wei;Licui Long;Chi Wang;Yonggui Wu
中科院分区：
文献类型：
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作者：
Yu-Fen He;Youfa Luo;C. Wei;Licui Long;Chi Wang;Yonggui Wu
利用有机改良剂辅助植物稳定化高含量重金属废渣是原位控制重金属释放的重要途径。然而，溶解性有机质（DOM）来自有机改良剂对HMs和微生物群落动态的影响仍然不清楚。在这里，DOM来自有机添加剂（牛粪）的HMs的地球化学行为和汞（Hg）-铊（Tl）采矿废渣中的细菌群落动态的影响进行了研究。结果表明，不添加DOM的汞铊采矿废渣随培养时间的延长，渗滤液pH值持续下降，EC、Eh、SO 42-、Hg和Tl含量持续升高。DOM的添加显著增加了pH、EC、SO 42-和砷（As）的含量，但降低了Eh、Hg和Tl的含量。DOM的添加显著增加了细菌群落的多样性和丰富度。优势细菌门（变形菌门、厚壁菌门、酸杆菌门、放线菌门和拟杆菌门）和属（芽孢杆菌、不动杆菌、代尔夫特氏菌、鞘氨醇单胞菌和肠杆菌）随着DOM含量和培养时间的增加而改变。渗滤液中的DOM组分均为类腐殖物质（C1和C2），且随着培养时间的延长，C1和C2的DOC含量和最大荧光强度（FMax）值呈先减小后增大再减小的趋势。HMs与DOM和细菌群落的相关性表明，HMs在汞铊采矿废渣中的地球化学行为受DOM介导的性质直接影响，并受DOM调节细菌群落变化的间接影响。总体而言，这些结果表明，DOM属性与细菌群落的变化增加了作为动员，但减少汞和铊动员汞铊采矿废渣。
Organic amendments in aided phytostabilization of waste slag containing high levels of heavy metal (loid)s (HMs) are an important way to control the release of HMsin situ. However, the effects of dissolved organic matter (DOM) derived from organic amendments on HMs and microbial community dynamics in waste slag are still unclear. Here, the effect of DOM derived from organic amendments (cow manure) on the geochemical behaviour of HMs and the bacterial community dynamics in mercury (Hg)-thallium (Tl) mining waste slag were investigated. The results showed that the Hg-Tl mining waste slag without the addition of DOM continuously decreased the pH and increased the EC, Eh, SO42−, Hg, and Tl levels in the leachate with increasing incubation time. The addition of DOM significantly increased the pH, EC, SO42−, and arsenic (As) levels but decreased the Eh, Hg, and Tl levels. The addition of DOM significantly increased the diversity and richness of the bacterial community. The dominant bacterial phyla (Proteobacteria, Firmicutes, Acidobacteriota, Actinobacteriota,andBacteroidota) and genera (Bacillus, Acinetobacter, Delftia, Sphingomonas,andEnterobacter) were changed in association with increases in DOM content and incubation time. The DOM components in the leachate were humic-like substances (C1 and C2), and the DOC content and maximum fluorescence intensity (FMax) values of C1 and C2 in the leachate decreased and first increased and then decreased with increasing incubation time. The correlations between HMs and DOM and the bacterial community showed that the geochemical behaviours of HMs in Hg-Tl mining waste slag were directly influenced by DOM-mediated properties and indirectly influenced by DOM regulation of bacterial community changes. Overall, these results indicated that DOM properties associated with bacterial community changes increased As mobilization but decreased Hg and Tl mobilization from Hg-Tl mining waste slag.