Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea
Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea
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DOI：
10.1007/s11262-007-0096-x
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发表时间：
2007-10-01
期刊：
影响因子：
1.6
通讯作者：
Park, Bong-Kyun
中科院分区：
文献类型：
--
作者：
Park, Seong-Jun;Moon, Hyoung-Joon;Park, Bong-Kyun
猪流行性腹泻病毒（PEDV）在韩国引起具有急性腹泻、脱水和显著死亡的毁灭性肠道疾病，从而引起严重的经济损失。刺突（S）糖蛋白已被认为是PEDV生物学特性的重要决定因素。在这项研究中，韩国PEDV分离株的部分S糖蛋白基因的核苷酸和推导的氨基酸序列，包括能够诱导PEDV中和抗体的表位区域，进行了测定。用RT-PCR方法扩增了PEDV S糖蛋白基因的部分片段，并进行了克隆、测序，同时与PEDV参考株进行了比较。通过系统发育分析，将韩国PEDV分离株分为G1、G2、G3三个类群，再分为G1-1、G1-2、G1-3三个亚群。组1（G1）与CV 777、Br 1/87、JS-2004-2、KPED-9、P-5V、SM 98 -1、亲本DR 13和减毒DR 13高度同源;组2（G2）与Spk 1高度同源;组3（G3）与Chinju 99高度同源。另外，韩国PEDV G1分离物中没有几个核苷酸和氨基酸序列与韩国PEDV G2和G3分离物相同，尤其是G1-1分离物中含有韩国PEDV G1-2、G1-3、G2和G3分离物中没有的核苷酸和氨基酸序列。提示许多韩国PEDV分离物与G1（包括CV 777、Br 1/87、JS-2004-2、KPED-9、P-5 V、SM 98 -1、亲本DR 13和减毒DR 13）密切相关，而与G2和G3（包括Spk 1和Chinju 99）不密切相关，并且在韩国分离的显著更流行的PEDV特别接近中国PEDV毒株JS-2004-2，而不是韩国PEDV毒株Spk 1、Chinju 99、KPED-9、SM 98 -1，亲本DR 13，和减毒的DR 13。
Porcine epidemic diarrhea virus (PEDV) causes a devastating enteric disease with acute diarrhea, dehydration and significant mortality in swine, thereby incurring heavy economic losses in Korea. Spike (S) glycoprotein has been suggested as an important determinant for PEDV biological properties. In this study, the nucleotide and deduced amino acid sequences of the partial S glycoprotein genes of Korean PEDV isolates, including epitope region that is capable of inducing PEDV-neutralizing antibodies, were determined. The partial S glycoprotein genes were amplified by RT-PCR, cloned, sequenced, and compared with each other as well as with reference PEDV strains. By phylogenetic analysis, the Korean PEDV isolates were divided into three groups (G1, G2, G3), which had three subgroups (G1-1, G1-2, G1-3). Group1 (G1) Korean PEDV isolates were highly homologous to CV777, Br1/87, JS-2004-2, KPED-9, P-5V, SM98-1, parent DR13, and attenuated DR13, group2 (G2) Korean PEDV isolates were highly homologous to Spk1, and group3 (G3) was Chinju99 at the nucleotide and deduced amino acid sequence levels. In addition, the G1 Korean PEDV isolates didn't had several specific nucleotides and amino acids which were found in the G2 and G3 Korean PEDV isolates, and especially the G1-1 Korean PEDV isolates had specific nucleotides and amino acids which were not found in the G1-2, G1-3, G2, and G3 Korean PEDV isolates. It was suggested that many Korean PEDV isolates are closely related to the G1 including CV777, Br1/87, JS-2004-2, KPED-9, P-5 V, SM98-1, parent DR13, and attenuated DR13 rather than to the G2 and G3 including Spk1 and Chinju99, and notably more prevalent PEDVs isolated in Korea are especially close to the Chinese PEDV strain JS-2004-2 rather than Korean PEDV strains Spk1, Chinju99, KPED-9, SM98-1, parent DR13, and attenuated DR13.