On-chip CO2 incubation for pocket-sized microfluidic cell culture
On-chip CO2 incubation for pocket-sized microfluidic cell culture
复制标题
用于袖珍微流体细胞培养的片上 CO2 孵育
DOI：
10.1007/s10404-011-0925-z
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发表时间：
2012
影响因子：
2.8
通讯作者：
N. Futai
中科院分区：
文献类型：
--
作者：
A. Takano;M. Tanaka;N. Futai
我们已经开发了一个芯片上的CO2孵育系统的基础上的质量/热量传递从碳酸氢盐源的水溶液的细胞培养基通过可渗透的聚二甲基硅氧烷（PDMS）壁。加热碳酸盐缓冲的碳酸氢盐溶液成功地调节了CO2的产生，而没有任何反馈控制。因为具有孵育系统的微流体细胞培养芯片不需要外部腔室或气体供应，所以整个微流体细胞培养装置变成口袋大小。使用5 ml 0.8 M碳酸氢钠和65 mM碳酸钠作为水套，当芯片置于37°C表面时，芯片将750 μl细胞培养基的温度、渗透压摩尔浓度和pH值保持在生理水平内。在至少9天内，培养基储库的渗透压变化和pCO 2稳定在<5 mmol/kg和5.0 ± 1.0%范围内。通过在倒置显微镜下微流控培养COS-7上皮细胞17天来证明孵育能力。
We have developed an on-chip CO2incubation system based on mass/heat transfer from aqueous solutions of bicarbonate source to cell culture media through a permeable poly(dimethylsiloxane) (PDMS) wall. Heating a carbonate-buffered bicarbonate solution successfully regulated CO2generation without any feedback control. Because a microfluidic cell culture chip with the incubation system does not require an external chamber or gas supply, the entire microfluidic cell culture setup becomes pocket sized. Using 5 ml of 0.8 M sodium bicarbonate with 65 mM sodium carbonate as the water jacket, the chip maintained the temperature, osmolality, and pH of 750 μl cell culture medium within physiological levels when the chip was placed on a 37°C surface. The osmolality shift and pCO2of the media reservoir stabilized within <5 mmol/kg and 5.0 ± 1.0% over at least 9 days. The incubation capabilities were demonstrated through microfluidic culture of COS-7 epithelial cells under an inverted microscope for 17 days.