Suppression of MED19 expression by shRNA induces inhibition of cell proliferation and tumorigenesis in human prostate cancer cells
Suppression of MED19 expression by shRNA induces inhibition of cell proliferation and tumorigenesis in human prostate cancer cells
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shRNA 抑制 MED19 表达可抑制人前列腺癌细胞的细胞增殖和肿瘤发生
DOI：
10.5483/bmbrep.2011.44.8.547
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发表时间：
2011-08-31
期刊：
影响因子：
3.8
通讯作者：
Yu, Hongyu
中科院分区：
文献类型：
--
作者：
Cui, Xingang;Xu, Danfeng;Yu, Hongyu
MED 19是介体的一员，在肿瘤中信号转导的激活和抑制或转录调节中起关键作用。为了检测MED 19在人前列腺癌中的功能作用，我们通过慢病毒介导的短发夹（shRNA）下调前列腺癌细胞（PC-3和DU 145）中MED 19的表达，并分析抑制MED 19对前列腺癌细胞增殖和肿瘤发生的影响。在MED 19下调后，体外前列腺癌细胞增殖、集落形成和裸鼠异种移植物中的体内肿瘤生长显著减少。MEDI 9的敲除引起S期停滞，并通过调节Bid和Caspase 7诱导细胞凋亡。MED 19被认为是一种新的增殖调节剂，可促进前列腺癌细胞的生长。[BMB报告2011; 44（8）：547-552]
MED19 is a member of the Mediator that plays a key role in the activation and repression of signal transduction or the regulation of transcription in carcinomas. To tested the functional role of MED19 in human prostate cancer, we downregulated MED19 expression in prostate cancer cells (PC-3 and DU145) by lentivirus-mediated short hairpin (shRNA), and analyzed the effect of inhibition of MED19 on prostate cancer cell proliferation and tumorigenesis. The in vitro prostate cancer cell proliferation, colony formation, and in vivo tumor growth in nude mice xenografts was significantly reduced after the downregulation of MED19. Knockdown of MEDI 9 caused S-phase arrest and induced apoptosis via modulation of Bid and Caspase 7. It was suggested that MED19 serves as a novel proliferation regulator that promotes growth of prostate cancer cells. [BMB reports 2011; 44(8): 547-552]