SCHISTOSOMA-MANSONI AND SCHISTOSOMA-HAEMATOBIUM - DIFFERENCES IN DEVELOPMENT
SCHISTOSOMA-MANSONI AND SCHISTOSOMA-HAEMATOBIUM - DIFFERENCES IN DEVELOPMENT
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DOI：
10.1016/0014-4894(81)90039-4
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发表时间：
1981-01-01
影响因子：
2.1
通讯作者：
UBELAKER, JE
中科院分区：
文献类型：
--
作者：
BURDEN, CS;UBELAKER, JE
生长和成熟的波多黎各菌株的S。mansoni和S.在感染后19天开始比较仓鼠中的嗜血杆菌。In S. mansoni，确定最佳发育，在第25天首次观察到交配，在第28天观察到卵壳蛋白质形成，在第30天产卵。在S.第一次交配发生在第29天。蛋壳蛋白在第45天形成，第60天产蛋。单性和两性感染的检查表明，成熟的卵黄可以更容易地评估自发荧光的蛋白质球比传统的重氮盐染色。自体荧光的活蠕虫与成熟的卵黄允许随后的检查EM和允许在亚细胞水平的评价。
Growth and maturation of the Puerto Rico strain of S. mansoni in mice and the Ghana strain of S. haematobium in hamsters were compared beginning 19 days after infection. In S. mansoni, optimum development was determined, with copulation first observed on day 25, egg shell protein formation observed day 28 and oviposition occurring on day 30. In infections of S. haematobium, copulation first occurred on day 29. Egg shell proteins were first formed on day 45 and egg production occurred on day 60. Examination of unisexual and bisexual infections showed that maturation of the vitellaria can be more easily assessed by autofluorescence of the protein globules than by the traditional diazonium salt stains. Autofluorescence of living worms with mature vitellaria allows subsequent examination by EM and permits evaluation at a subcellular level.