Decreased Aperture Surface Energy Enhances Electrical, Mechanical, and Temporal Stability of Suspended Lipid Membranes
Decreased Aperture Surface Energy Enhances Electrical, Mechanical, and Temporal Stability of Suspended Lipid Membranes
复制标题
DOI：
10.1021/am403605h
复制
发表时间：
2013-11-27
影响因子：
9.5
通讯作者：
Aspinwall, Craig A.
中科院分区：
文献类型：
--
作者：
Bright, Leonard K.;Baker, Christopher A.;Aspinwall, Craig A.
下一代跨膜蛋白生物传感器的开发在很大程度上依赖于黑脂膜（BLMs）的使用；然而，blm的电、机械和时间不稳定性对生物传感器的发展提出了限制挑战。在这项工作中，用不同链长和氟组成的硅烷修饰微米尺寸的玻璃孔，包括3-氰丙基二甲基氯硅烷（CPDCS）、乙基二甲基氯硅烷（EDCS）、正辛基二甲基氯硅烷（ODCS）、（三氟-1,1,2,2-四氢辛基）二甲基氯硅烷（PFDCS）或（十六氟-1,1,2,2-四氢癸基）二甲基氯硅烷（PFDDCS），以探索底物表面能对BLM稳定性的影响。低能量硅烷修饰的表面促进了脂质底物的相互作用，促进了低泄漏，稳定的blm的形成。CDCS、EDCS、ODCS、PFDCS和PFDDCS的表面能分别为30 +/- 3、16 +/- 1、14 +/- 2、11 +/- 1和7.1 +/- 2 mj m（-2）。表面能的降低与电、机械和时间BLM稳定性的提高直接相关。与传统的碳氢化合物改性剂相比，两疏全氟表面改性剂在稳定性和BLM形成方面具有优越的性能，对BLM膜透性只有边际影响。PFDCS和PFDDCS blm的泄漏电流仅提高了10-30%，尽管与先前报道的CPDCS改造相比，PFDDCS改造产生的击穿电压（> 2000 mV vs 418 +/- 73 mV）表明电气稳定性提高了>5倍，空气-水转移（> 50 vs 2 +/- 0.2）表明机械稳定性提高了>25倍。重要的是，正如α -溶血素活性所证明的那样，在没有对重构离子通道功能产生有害影响的情况下，显著提高了膜的稳定性。因此，这种方法为BLM稳定提供了一种简单、低成本、广泛适用的替代方案，并将对下一代离子通道固定化生物传感器的发展做出重大贡献。
The development of next-generation transmembrane protein-based biosensors relies heavily on the use of black lipid membranes (BLMs); however, electrical, mechanical, and temporal instability of BLMs poses a limiting challenge to biosensor development. In this work, micrometer-sized glass apertures were modified with silanes of different chain length and fluorine composition, including 3-cyanopropyldimethychlorosilane (CPDCS), ethyldimethylchlorosilane (EDCS), n-octyldimethylchlorosilane (ODCS), (tridecafluoro-1, 1, 2, 2-tetrahydrooctyl)dimethylchlorosilane (PFDCS), or (heptadecafluoro-1,1,2,2-tetrahydrodecyl)dimethylchlorosilane (PFDDCS), to explore the effect of substrate surface energy on BLM stability. Low energy silane-modified surfaces promoted enhanced lipid substrate interactions that facilitate the formation of low-leakage, stable BLMs. The surface energies of silane-modified substrates were 30 +/- 3, 16 +/- 1, 14 +/- 2, 11 +/- 1, and 7.1 +/- 2 mj m(-2) for CDCS, EDCS, ODCS, PFDCS, and PFDDCS, respectively. Decreased surface energy directly correlated to improved electrical, mechanical, and temporal BLM stability. Amphiphobic perfluorinated surface modifiers yielded superior performance compared to traditional hydrocarbon modifiers in terms of stability and BLM formation, with only marginal effects on BLM membrane permeability. Leakage currents obtained for PFDCS and PFDDCS BLMs were elevated only 10-30%, though PFDDCS modification yielded >5-fold increase in electrical stability as indicated by breakdown voltage (> 2000 mV vs 418 +/- 73 mV), and >25-fold increase in mechanical stability as indicated by air-water transfers (> 50 vs 2 +/- 0.2) when compared to previously reported CPDCS modification. Importantly, the dramatically improved membrane stabilities were achieved with no deleterious effects on reconstituted ion channel function, as evidenced by alpha-hemolysin activity. Thus, this approach provides a simple, low cost, and broadly applicable alternative for BLM stabilization and should contribute significantly toward the development of next-generation ion-channel-fiinctionalized biosensors.