Crosslinking of the cell surface immunoglobulin (mu-surrogate light chains complex) on pre-B cells induces activation of V gene rearrangements at the immunoglobulin kappa locus.
Crosslinking of the cell surface immunoglobulin (mu-surrogate light chains complex) on pre-B cells induces activation of V gene rearrangements at the immunoglobulin kappa locus.
复制标题
前 B 细胞上细胞表面免疫球蛋白（mu 替代轻链复合物）的交联可诱导免疫球蛋白 kappa 位点处 V 基因重排的激活。
作者：
T. Tsubata;R. Tsubata;M. Reth
我们构建了编码缺少VH和CH 1结构域的截短的IG μ链（μ δ m链）的表达载体，并将μ δ m载体引入IG阴性Abelson前B细胞系P17-27中。转染子在其表面上表达大量的μ δ m链，其不与λ 5和VpreB替代轻链分子复合。虽然用完整微米链的载体转染的P17-27（P17-27微米）在培养物中显示V κ重排，但在P17-27 μ Δ m细胞中未检测到V κ重排。当细胞表面的μ δ m链被抗μ抗体交联时，在P17-27 μ δ m中诱导V κ基因重排。这些结果有力地表明，前B细胞表面上的微米-λ 5-VpreB复合物的交联产生激活V κ基因重排的信号，并且λ 5和VpreB分子对于前B细胞上的表面IG的自发交联是必需的。
We constructed an expression vector encoding a truncated Ig mu chain that lacks both VH and CH1 domains (mu delta m chain) and introduced the mu delta m vector into the Ig negative Abelson pre-B cell line P17-27. The transfectants expressed a large amount of the mu delta m chain on their surface, which was not complexed with the lambda 5 and VpreB surrogate light chain molecules. While P17-27 transfected with a vector for the intact micron chain (P17-27 micron) shows V kappa rearrangements in culture, V kappa rearrangements were not detected in P17-27 mu delta m cells. When the mu delta m chains on the cell surface were crosslinked by anti-mu antibodies, V kappa gene rearrangements were induced in P17-27 mu delta m. These results strongly suggest that crosslinking of the micron-lambda 5-VpreB complex on the pre-B cell surface generates a signal that activates V kappa gene rearrangement, and that the lambda 5 and VpreB molecules are necessary for the spontaneous crosslinking of surface Ig on pre-B cells.