Response surface optimization of enzymatic hydrolysis of Cistus ladanifer and Cytisus striatus for bioethanol production
Response surface optimization of enzymatic hydrolysis of Cistus ladanifer and Cytisus striatus for bioethanol production
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DOI：
10.1016/j.bej.2009.03.012
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发表时间：
2009-08-15
影响因子：
3.9
通讯作者：
Domingues, Fernanda C.
中科院分区：
文献类型：
--
作者：
Ferreira, Susana;Duarte, Ana P.;Domingues, Fernanda C.
目前使用甘蔗和玉米等作物的乙醇生产工艺已经很成熟：然而，利用更便宜的底物如木质纤维素可以使生物乙醇与化石燃料更具竞争力，而不会产生与使用潜在粮食资源相关的伦理问题。用于获得纤维素乙醇的顺序配置意味着预处理的木质纤维素材料的固体部分经历水解。在这项工作中，酶水解预处理岩蔷薇和红蜘蛛条纹以下的实验设计作为一个统计问题解决方法进行了研究。采用Plackett-Burman试验设计对酶解工艺进行优化，同时考察了操作条件和反应条件对酶解的影响，采用中心组合试验设计对酶解工艺进行优化，并采用响应面法对酶解工艺进行优化（pH、温度、纤维素酶浓度、聚合物（PEG）浓度和孵育时间）和由于所用底物类型（C ladanifer和C striatus）的可变性。从所获得的结果可以得出结论，酶水解明显增强温度，纤维素酶浓度，和孵育时间。模型验证表明，实验结果和预测的响应之间有很好的协议。(C)2009 Elsevier B. V.保留所有权利。
Current ethanol production processes using crops such as sugar cane and corn are well established: however, utilization of a cheaper substrate such as lignocellulose could make bioethanol more competitive with fossil fuel, without the ethical concerns associated with the use of potential food resources. The sequential configuration employed to obtain cellulosic ethanol implies that the solid fraction of pretreated lignocellulosic material undergoes hydrolysis. In this work, the enzymatic hydrolysis of pretreated Cistus ladanifer and Cytisus striatus was studied following an experimental design as a statistical problem solving approach. Plackett-Burman design was used in order to select the most important variables from the simultaneous study on influence of operating and reactional conditions, and central composite design to optimize the process of enzymatic hydrolysis.The optimization of enzymatic hydrolysis using the response surface methodology allowed a study on the influence of the variables (pH, temperature, cellulases concentration, polymer (PEG) concentration and incubation time) and variability due to the type of substrate (C ladanifer and C striatus) used. From the obtained results it can be concluded that the enzymatic hydrolysis was clearly enhanced by temperature, cellulase concentration, and incubation time. Model validation showed a good agreement between experimental results and the predicted responses. (C) 2009 Elsevier B.V. All rights reserved.