The GPIIB-IIIa-like complex may function as a human melanoma cell adhesion receptor for thrombospondin.
The GPIIB-IIIa-like complex may function as a human melanoma cell adhesion receptor for thrombospondin.
复制标题
GPIIB-IIIa 样复合物可能充当血小板反应蛋白的人黑色素瘤细胞粘附受体。
DOI：
10.1016/0014-4827(89)90251-6
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发表时间：
1989
影响因子：
3.7
通讯作者：
Knudsen,KA
中科院分区：
文献类型：
--
作者：
Tuszynski,GP;Karczewski,J;Smith,L;Murphy,A;Rothman,VL;Knudsen,KA
本研究的目的是确定与纤维蛋白原受体免疫相关的异二聚体复合体是否可以在TSP介导的人黑色素瘤细胞-基质黏附中发挥TSP受体的作用。我们发现，抗血小板GPIIb-IIIa复合体GPIIIa和人Vitronectin受体的多克隆抗体可抑制TSP介导的细胞黏附63%-68%。用抗人血小板GPIIb-II1a或抗人Vitronectin受体抗体免疫沉淀125I标记黑色素瘤细胞的洗涤剂提取物，发现存在单一的异二聚体复合体，表明这两种抗血清识别相同的整合素受体，即GPIIb-IIIa样抗原。细胞与TSP的黏附可能是通过TSP分子中含有精氨酸-甘氨酸-天冬氨酸(RGD)肽序列的区域来调节的，因为在含有RGD的多肽存在下，细胞与TSP的黏附被抑制了50%-66%。这些结果有力地表明，GPIIb-IIIa样受体/Vitronectin受体可以作为TSP在介导细胞-基质黏附中的细胞结合部位。
The purpose of this study was to determine whether a heterodimeric complex immunologically related to the fibrinogen receptor could function as a thrombospondin (TSP) receptor in TSP-mediated cell-substratum adhesion of human melanoma cells. We found that polyclonal antibodies to the platelet GPIIb-IIIa complex, GPIIIa, and the human vitronectin receptor inhibited TSP-mediated cell adhesion by 63–68%. Immunoprecipitation of detergent extracts of125I-surface-labeled melanoma cells using either anti-human platelet GPIIb-IIla or anti-human vitronectin receptor antibody revealed the presence of a single heterodimeric complex, suggesting that both antisera recognize the same integrin receptor, GPIIb-IIIa-like antigen. Adhesion of cells to TSP is likely mediated through a region of the TSP molecule containing the arginine-glycine-aspartic (RGD) peptide sequence, since cell attachment to TSP was inhibited 50–66% in the presence of peptides containing RGD. These results strongly suggest that a GPIIb-IIIa-like/vitronectin receptor can serve as a cell binding site for TSP in mediating cell-substratum adhesion.