INHERITANCE OF ANTIBODY SPECIFICITY
INHERITANCE OF ANTIBODY SPECIFICITY
复制标题
抗体特异性的遗传
DOI：
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发表时间：
1974
影响因子：
15.3
通讯作者：
O. Mäkelä
中科院分区：
文献类型：
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作者：
T. Imanishi;O. Mäkelä
我们的数据表明，抗半抗原抗体的良好特异性是可变(V)基因的一个有用的孟德尔标记。我们发现，一些小鼠菌株（如C57/BL6）一致产生异位抗（4-羟基-3-硝基苯基）乙酰（NP）抗体（相关的（4-羟基-5-碘-3-硝基苯基）乙酰和（4-羟基-3.5-二硝基苯基）乙酰的相对亲和力始终为bb0 2），而其他菌株（如CBA）产生“常规”抗NP抗体（相对亲和力始终<1）。48只（CBA × C57BL/6）F1小鼠均有异位抗np抗体。将它们回交到隐性CBA亲本，并对87只回交动物进行了类似的测试。C57BL/6重(H)链同种型的杂合性与C57BL/6和F1小鼠相似，而CBA同种型的纯合性与CBA小鼠无明显区别。这种单基因遗传仅在原发性反应中观察到。通过免疫选择的纯合子小鼠株，证实了同种异型连锁基因在控制精细特异性特性方面的优势。这些小鼠含有来自C57BL、BALB/c和其他菌株的各种基因混合物。它们的抗np特异性完全由h链等位型相关基因决定，不受包括h -2连锁基因在内的其他基因的影响。
Our data suggest that fine specificity of antihapten antibodies is a useful Mendelian marker of variable (V) genes. We found that some mouse strains (e.g., C57/BL6) consistently produced heteroclitic anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies (relative affinity for related (4-hydroxy-5-iodo-3-nitrophenyl)acetyl and (4-hydroxy-3.5-dinitrophenyl)acetyl was always >2) while other strains (e.g., CBA) produced "conventional" anti-NP antibodies (relative affinities were consistently <1). 48 (CBA x C57BL/6)F1 mice were studied and most of them had heteroclitic anti-NP antibodies. They were backcrossed to the recessive CBA parent, and 87 backcross animals were similarly tested. Those heterozygous for the C57BL/6 heavy (H)-chain allotype were similar to the C57BL/6 and the F1 mice while mice homozygous for the CBA allotype were indistinguishable from the CBA. Such monogenic inheritance was observed only in the primary response. Predominance of allotype-linked genes in the control of the fine specificity characteristics was confirmed by immunizing selected homozygous mouse strains. These mice contained various mixtures of genes from C57BL, BALB/c, and other strains. Specificity of their anti-NP was exclusively determined by genes linked to the H-chain allotype, no influence could be attributed to other genes including the H-2-linked genes.