Bringing back the fruit into fruit fly-bacteria interactions
Bringing back the fruit into fruit fly-bacteria interactions
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DOI：
10.1111/j.1365-294x.2008.03674.x
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发表时间：
2008-03-01
影响因子：
4.9
通讯作者：
Yuval, B.
中科院分区：
文献类型：
--
作者：
Behar, A.;Jurkevitch, E.;Yuval, B.
雌性地中海果蝇（Ceratitis headata）在果实中产卵，幼虫在果实中发育。这种发展与水果的快速恶化有关，并且经常与次要害虫的入侵有关。大多数关于地中海果蝇和微生物之间关系的研究都集中在成蝇消化系统中的细菌上，而果实在介导、放大或调节果蝇微生物区系中的作用在很大程度上被忽视了。在这项研究中，我们研究了这样一个假设：宿主果实在维持果蝇相关细菌群落的过程中发挥着作用。使用直接和基于培养的方法，我们表明该群落由绝大多数固氮和果胶分解肠杆菌科细菌组成。我们的数据表明，这种与果蝇相关的肠细菌群落是从母本垂直传播到其后代的。在产卵过程中，细菌被转移到果实上，在其中定居并增殖，导致果实腐烂。这些结果表明，宿主水果确实是果蝇与细菌相互作用的核心伙伴，因为这些传播的细菌被水果放大，并随后在果蝇的整个生命中得以维持。这种肠细菌群落可能有助于果蝇的氮和碳代谢，影响其发育并最终影响其健康。
Female Mediterranean fruit flies (Ceratitis capitata) oviposit in fruits, within which the larvae develop. This development is associated with rapid deterioration of the fruit, and frequently with invasion by secondary pests. Most research on the associations between medflies and microorganisms has focused on the bacteria inhabiting the digestive system of the adult fly, while the role of the fruit in mediating, amplifying or regulating the fruit fly microflora has been largely neglected. In this study, we examine the hypothesis that the host fruit plays a role in perpetuating the fly-associated bacterial community. Using direct and cultured-based approaches, we show that this community is composed in its very large majority of diazotrophic and pectinolytic Enterobacteriaceae. Our data suggest that this fly-associated enterobacterial community is vertically transmitted from the female parent to its offspring. During oviposition, bacteria are transferred to the fruit, establish and proliferate within it, causing its decay. These results show that the host fruit is indeed a central partner in the fruit fly-bacterial interaction as these transmitted bacteria are amplified by the fruit, and subsequently maintained throughout the fly's life. This enterobacterial community may contribute to the fly's nitrogen and carbon metabolism, affecting its development and ultimately, fitness.