Evaluation of a time efficient immunization strategy for anti-PAH antibody development.
Evaluation of a time efficient immunization strategy for anti-PAH antibody development.
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评估抗PAH抗体开发的时间有效免疫策略。
DOI：
10.1080/15321819.2016.1198803
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发表时间：
2016
影响因子：
--
通讯作者：
Unger MA
中科院分区：
文献类型：
--
作者：
Li X;Kaattari SL;Vogelbein MA;Unger MA
开发对小分子具有亲和力的单克隆抗体（mAb）可能是一个耗时的过程。为了评估mAb生产时间的缩短，我们在免疫后的不同时间点检查小鼠抗血清以测量滴度并评估对免疫原PBA（芘丁酸）的亲和力。还暂时进行融合以评价在不同时间段的抗体产生成功。我们在免疫后7周产生抗PBA抗体，并在杂交瘤筛选过程中选择抗PAH反应性。此外，相对于从更传统的18周时间表筛选的抗体，没有明显的灵敏度差异。我们的研究结果表明，一个更有效的时间免疫策略抗PAH抗体的发展，可适用于其他小分子。
The development of monoclonal antibodies (mAb) with affinity to small molecules can be a time-consuming process. To evaluate shortening the time for mAb production, we examined mouse antisera at different time points post-immunization to measure titer and to evaluate the affinity to the immunogen PBA (pyrene butyric acid). Fusions were also conducted temporally to evaluate antibody production success at various time periods. We produced anti-PBA antibodies 7 weeks post-immunization and selected for anti-PAH reactivity during the hybridoma screening process. Moreover, there were no obvious sensitivity differences relative to antibodies screened from a more traditional 18 week schedule. Our results demonstrate a more time efficient immunization strategy for anti-PAH antibody development that may be applied to other small molecules.