Volumetric retinal imaging with ultrahigh-resolution spectral-domain optical coherence tomography and adaptive optics using two broadband light sources
Volumetric retinal imaging with ultrahigh-resolution spectral-domain optical coherence tomography and adaptive optics using two broadband light sources
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DOI：
10.1364/oe.17.004095
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发表时间：
2009-03-02
期刊：
影响因子：
3.8
通讯作者：
Miller, Donald T.
中科院分区：
文献类型：
--
作者：
Cense, Barry;Koperda, Eric;Miller, Donald T.
超宽带光源，如多路超辐射二极管(SLD)和飞秒激光器，已经成功地应用于用于超高分辨率视网膜成像的自适应光学相干层析成像(AO-OCT)系统。然而，这些来源的成本差异很大，因此需要进行业绩比较。在这里，我们比较了飞秒激光器的整体钛宝石激光器和超光宽光T840的性能，使用相同的AO-OCT系统和相同的对象。此外，我们还研究了配备了积分器的仪器在第二个对象上捕获中心凹和邻近区域的体积图像的能力，使用AO来控制视网膜的焦点，并使用定制和免费的图像配准软件来减少眼部运动伪影。单色眼球差用低音扬声器-高音声学系统矫正。分别在3.2微米和3.3微米处对整体式和宽光式光源的相干长度进行了体内测量。动态范围的差异为5分贝，接近实验的预期变异性。个体视锥感光细胞、视网膜毛细血管和神经纤维束在这两个来源的所有三个维度上都被区分开来。获取的视网膜体积在OSA ISP中提供以供查看，允许读者在显微镜水平上进行数据挖掘。(C)2009年美国光学学会
Ultrabroadband sources, such as multiplexed superluminescent diodes (SLDs) and femtosecond lasers, have been successfully employed in adaptive optics optical coherence tomography (AO-OCT) systems for ultrahigh resolution retinal imaging. The large cost differential of these sources, however, motivates the need for a performance comparison. Here, we compare the performance of a Femtolasers Integral Ti:Sapphire laser and a Superlum BroadLighter T840, using the same AO-OCT system and the same subject. In addition, we investigate the capability of our instrument equipped with the Integral to capture volume images of the fovea and adjacent regions on a second subject using the AO to control focus in the retina and custom and freeware image registration software to reduce eye motion artifacts. Monochromatic ocular aberrations were corrected with a woofer-tweeter AO system. Coherence lengths of the Integral and BroadLighter were measured in vivo at 3.2 mu m and 3.3 mu m, respectively. The difference in dynamic range was 5 dB, close to the expected variability of the experiment. Individual cone photoreceptors, retinal capillaries and nerve fiber bundles were distinguished in all three dimensions with both sources. The acquired retinal volumes are provided for viewing in OSA ISP, allowing the reader to data mine at the microscope level. (C) 2009 Optical Society of America