Screening for new hydroxynitrilases from plants
Screening for new hydroxynitrilases from plants
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DOI：
10.1271/bbb.69.2349
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发表时间：
2005-12-01
影响因子：
1.6
通讯作者：
Ohmiya, T
中科院分区：
文献类型：
--
作者：
Asano, Y;Tamura, K;Ohmiya, T
建立了一种简单的HPLC法测定苯甲醛和氰化物合成的手性扁桃腈的活性和立体化学，并将其应用于植物源羟基腈裂解酶（HNL）活性的筛选。用该方法检测了74科163种植物的（R）-和（S）-HNL活性。我们发现，Baliospermum montanum的叶匀浆显示出（S）HNL活性，而西番莲的叶和种子，以及枇杷、木瓜、花楸、梅和桃的种子显示出（R）-HNL活性。从西番莲和枇杷中部分纯化的（R）HNL不仅作用于苯甲醛，而且作用于脂肪酮。以西番莲叶匀浆为原料，由2-戊酮合成的（R）-甲基丙基酮氰醇的对映体过量为87.0%，以枇杷籽匀浆为原料合成的（R）-扁桃腈的对映体过量为85.0%。
We established a simple HPLC method to determine the activity and stereochemistry of the chiral mandelonitrile synthesized from benzaldehyde and cyanide, and applied it to screen for hydroxynitrile lyase (HNL) activity of plant origin. A total of 163 species of plants among 74 families were examined for (R)- and (S)-HNL activities using the method. We discovered that homogenate of leaves of Baliospermum montanum shows (S)HNL activity, while leaves and seeds from Passiflora edulis, and seeds from Eriobotrya japonica, Chaenomles sinensis, Sorbus anucuparia, Prunus mume, and Prunus persica show (R)-HNL activity. Partially purified (R)HNLs from Passiflora edulis and Eriobotrya japonica acted not only on benzaldehyde but also on aliphatic ketone. The enantiomeric excess of (R)-methylpropylketone cyanohydrin synthesized from 2-pentanone using homogenate from leaves of Passiflora edulis was 87.0%, and that of (R)-mandelonitrile synthesized by homogenate from seeds of Eriobotrya japonica was 85.0%.