Induction of oxidative stress and apoptosis by silver nanoparticles in the liver of adult zebrafish
Induction of oxidative stress and apoptosis by silver nanoparticles in the liver of adult zebrafish
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DOI：
10.1016/j.aquatox.2009.12.012
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发表时间：
2010-10-15
影响因子：
4.5
通讯作者：
Ryu, Doug-Young
中科院分区：
文献类型：
--
作者：
Choi, Ji Eun;Kim, Soohee;Ryu, Doug-Young
银纳米颗粒（AgNPs）在环境释放过程中会对水生生物产生有害影响。银纳米颗粒的肝毒性在成年斑马鱼的肝脏中进行了评估，目的是研究氧化损伤和细胞凋亡的作用。将斑马鱼暴露于AgNP溶液中，其中在处理时不存在游离Ag+离子。然而，金属敏感的金属硫蛋白2（MT 2）的mRNA的银纳米粒子处理后的斑马鱼的肝组织中诱导，这表明银离子从银纳米粒子处理后释放。这也可能是MT 2 mRNA的诱导在肝组织中的银纳米粒子产生的自由基。在肝组织的组织学分析中观察到许多细胞改变，包括肝细胞索的破坏和凋亡变化。丙二醛，细胞脂质过氧化的副产物，和总谷胱甘肽的水平增加后，与银纳米粒子治疗的组织。组织中的氧自由基清除酶过氧化氢酶和谷胱甘肽过氧化物酶la的mRNA水平降低。AgNP处理诱导DNA损伤，如通过用双链断裂标记物γ-H2 AX和肝组织中p53蛋白表达的分析所证明的。此外，用AgNP处理后，p53相关的促凋亡基因Bax、Noxa和p21上调。这些数据表明，氧化应激和细胞凋亡与银纳米粒子在成年斑马鱼的肝脏毒性。(C)2009爱思唯尔有限公司版权所有。
Silver nanoparticles (AgNPs) may induce deleterious effects in aquatic life on environmental release. The hepatotoxicity of AgNPs was assessed in the liver of adult zebrafish, with the aim of studying the roles of oxidative damage and apoptosis. Zebrafish were exposed to an AgNP solution in which free Ag+ ions were absent at the time of treatment. However, the metal-sensitive metallothionein 2 (MT2) mRNA was induced in the liver tissues of AgNP-treated zebrafish, suggesting that Ag+ ions were released from AgNPs after treatment. It is also possible that MT2 mRNA was induced in the liver tissues by AgNP-generated free radicals. A number of cellular alterations including disruption of hepatic cell cords and apoptotic changes were observed in histological analysis of the liver tissues. The levels of malondialdehyde, a byproduct of cellular lipid peroxidation, and total glutathione were increased in the tissues after treatment with AgNPs. The mRNA levels of the oxyradical-scavenging enzymes catalase and glutathione peroxidase la were reduced in the tissues. AgNP treatment induced DNA damage, as demonstrated by analysis with the double-strand break marker gamma-H2AX and the expression of p53 protein in liver tissues. In addition, the p53-related pro-apoptotic genes Bax, Noxa, and p21 were upregulated after treatment with AgNPs. These data suggest that oxidative stress and apoptosis are associated with AgNP toxicity in the liver of adult zebrafish. (C) 2009 Elsevier B.V. All rights reserved.