Permittivity of ex vivo healthy and diseased murine skeletal muscle from 10 kHz to 1 MHz
Permittivity of ex vivo healthy and diseased murine skeletal muscle from 10 kHz to 1 MHz
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DOI：
10.1038/s41597-019-0045-2
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发表时间：
2019-04-18
期刊：
影响因子：
9.8
通讯作者：
Sanchez, B.
中科院分区：
文献类型：
--
作者：
Nagy, J. A.;DiDonato, C. J.;Sanchez, B.
更好地了解骨骼肌的介电常数特性对于开发新的诊断工具和神经肌肉评估方法至关重要。然而，在我们对健康和患病骨骼肌的这种性质的理解中仍然存在重要的知识差距，这阻碍了其转化为临床应用。在这里，我们报告腓肠肌的介电常数在健康的野生型小鼠和脊髓性肌萎缩症，肌营养不良症，糖尿病，肌萎缩侧索硬化症的小鼠模型和肌纤维肥大的模型。使用四电极阻抗技术从10 kHz至1 MHz离体测量数据。获得了额外的定量组织学信息。最终，报告的规范性数据将为科学界提供开发更准确模型的机会，用于验证和预测临床前和临床神经肌肉疾病研究中的实验观察结果。
A better understanding of the permittivity property of skeletal muscle is essential for the development of new diagnostic tools and approaches for neuromuscular evaluation. However, there remain important knowledge gaps in our understanding of this property in healthy and diseased skeletal muscle, which hinder its translation into clinical application. Here, we report the permittivity of gastrocnemius muscle in healthy wild type mice and murine models of spinal muscular atrophy, muscular dystrophy, diabetes, amyotrophic lateral sclerosis and in a model of myofiber hypertrophy. Data were measured ex vivo from 10 kHz to 1MHz using the four-electrode impedance technique. Additional quantitative histology information were obtained. Ultimately, the normative data reported will offer the scientific community the opportunity to develop more accurate models for the validation and prediction of experimental observations in both pre-clinical and clinical neuromuscular disease research.