Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo
Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo
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DOI：
10.1038/nm0901-1035
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发表时间：
2001-09-01
期刊：
影响因子：
82.9
通讯作者：
Mayer, JE
中科院分区：
文献类型：
--
作者：
Kaushal, S;Amiel, GE;Mayer, JE
动脉导管因动脉内皮细胞所具有的固有功能特性，尤其是在生理刺激下产生一氧化氮的能力，在外科搭桥手术中越来越受到青睐。在此我们测试了内皮祖细胞（EPCs）是否能够替代动脉内皮细胞并促进组织工程化小直径血管（4毫米）的通畅性。我们从绵羊外周血中分离出内皮祖细胞，在体外进行扩增，然后将其接种到去细胞化的猪髂血管上。接种了内皮祖细胞的移植物作为绵羊颈动脉间置移植物在130天内保持通畅，而未接种的移植物在15天内就发生了堵塞。接种内皮祖细胞的移植物表现出收缩活性以及由一氧化氮介导的血管舒张，这与天然颈动脉相似。这些结果表明内皮祖细胞能够起到与动脉内皮细胞相似的作用，从而使血管移植物具有更长的存活时间。由于其独特的性质，内皮祖细胞可能在组织工程化结构以及治疗血管疾病方面有其他普遍的应用。
Arterial conduits are increasingly preferred for surgical bypass because of inherent functional properties conferred by arterial endothelial cells, especially nitric oxide production in response to physiologic stimuli. Here we tested whether endothelial progenitor cells (EPCs) can replace arterial endothelial cells and promote patency in tissue-engineered small-diameter blood vessels (4 mm). We isolated EPCs from peripheral blood of sheep, expanded them ex vivo and then seeded them on decellularized porcine iliac vessels. EPC-seeded grafts remained patent for 130 days as a carotid interposition graft in sheep, whereas non-seeded grafts occluded within 15 days. The EPC-explanted grafts exhibited contractile activity and nitric-oxide-mediated vascular relaxation that were similar to native carotid arteries. These results indicate that EPCs can function similarly to arterial endothelial cells and thereby confer longer vascular-graft survival. Due to their unique properties, EPCs might have other general applications for tissue-engineered structures and in treating vascular diseases.