Differences in the Soluble, Residual Phosphate Concentrations at Which Coastal Phytoplankton Species Up-regulate Alkaline-Phosphatase Expression, as Measured By Flow-Cytometric Detection of ELF-97® Fluorescence
Differences in the Soluble, Residual Phosphate Concentrations at Which Coastal Phytoplankton Species Up-regulate Alkaline-Phosphatase Expression, as Measured By Flow-Cytometric Detection of ELF-97® Fluorescence
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DOI：
10.1007/s12237-009-9211-7
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发表时间：
2009-09
影响因子：
2.7
通讯作者：
S. Meseck;Jennifer H. Alix;G. Wikfors;J. Evan Ward
中科院分区：
文献类型：
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作者：
S. Meseck;Jennifer H. Alix;G. Wikfors;J. Evan Ward
酶标记荧光 (ELF-97®) 底物在碱性磷酸酶 (AP) 活性位点产生不溶性荧光黄绿色产物。对 15 种沿海浮游植物进行了测试，以确定是否可以在磷酸盐耗尽的介质中检测到 AP 活性。除杆菌聚球藻外，所有测试的物种均表达 AP 活性。随后，确定了 ELF-97® 可以检测到 AP 活性的可溶性活性磷酸盐 (SRP) 的阈值浓度，其中包括新小角毛藻、自养小球藻、等鞭金藻、最小原甲藻和 Tetraselmis chui。微藻物种在首次检测到 AP 活性的 SRP 浓度 (10.1–16.4 µM) 上存在显着差异，远高于通常认为的限制浓度。浮游植物在比其他藻类物种更高的 SRP 浓度下，minimum 开始表达 AP 活性；这可能归因于 P 中相对较高的 DNA/细胞比率。与其他浮游植物相比最低。因此，浮游植物物种可能会对高 SRP 浓度下的磷缺乏做出反应。
The enzyme-labeled fluorescence (ELF-97®) substrate produces an insoluble, fluorescent yellow-green product at the site of alkaline-phosphatase (AP) activity. Fifteen coastal phytoplankton species were tested to determine if AP activity could be detected in phosphate-depleted media. All species tested, exceptSynechococcus bacillaris, expressed AP activity. Subsequently, threshold concentrations of soluble reactive phosphate (SRP) at which AP activity could be detected by ELF-97® were determined forChaetoceros neogracile,Chlorella autotrophica,Isochrysissp.,Prorocentrum minimum, andTetraselmis chui.Microalgal species differed significantly in the SRP concentration at which AP activity was first detectable (10.1–16.4 µM), well above concentrations normally considered limiting for phytoplankton.P. minimumbegan to express AP activity at a higher SRP concentration than the other algal species; this may be attributable to a relatively high DNA/cell ratio inP. minimum, compared to the other phytoplankton. Thus, phytoplankton species may respond to phosphorus deficiency at high SRP concentrations.