EkFLS overexpression promotes flavonoid accumulation and abiotic stress tolerance in plant
EkFLS overexpression promotes flavonoid accumulation and abiotic stress tolerance in plant
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EkFLS 过表达促进植物类黄酮积累和非生物胁迫耐受性
DOI：
10.1111/ppl.13407
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发表时间：
2021
影响因子：
6.4
通讯作者：
Xia Cai
中科院分区：
文献类型：
--
作者：
Meng Wang;Yue Zhang;Chenyu Zhu;Xiangyu Yao;Zhe Zheng;Zheni Tian;Xia Cai
黄酮类化合物具有很高的药用价值，对植物个体的生长发育和抗逆性起着重要作用。黄酮醇合成酶（FLS）是合成黄酮类化合物的关键酶之一。然而，FLS基因在植物类黄酮积累和非生物胁迫耐受性中的作用及其机制尚未得到系统的研究。本研究的目的是评价FLSovere-expression对甘遂活性成分积累和抗逆性的影响。结果表明，在拟南芥中过量表达EkFLS基因后，黄酮类化合物的积累量增加。此外，当用阿坝和MeJA处理野生型和表达EkFLSovereen的拟南芥时，与WT拟南芥相比，表达EkFLSovereen的拟南芥促进气孔开度，从而影响植物的光合作用，这反过来又可以促进抗逆性。同时，在MeJA、NaCl和PEG处理下，拟南芥中表达的EkFLSovereen诱导了较高的类黄酮积累，从而显著提高了过氧化物酶（POD）和超氧化物歧化酶（SOD）的活性，使细胞内活性氧自由基减少，保护植物。这些结果表明，EkFLSovereen基因的表达与植物类黄酮合成的增加以及植物对非生物胁迫的耐受性密切相关，为进一步提高药用植物的品质同时提高其对非生物胁迫的耐受性提供了理论依据。
Flavonoids with great medicinal value play an important role in plant individual growth and stress resistance. Flavonol synthetase (FLS) is one of the key enzymes to synthesize flavonoids. However, the role of theFLSgene in flavonoid accumulation and tolerance to abiotic stresses, as well as its mechanism has not yet been investigated systematically in plants. The aim of this research is to evaluate the effect ofFLSoverexpression on the accumulation of active ingredients and stress resistance inEuphorbia kansuiLiou. The results showed that when theEkFLSgene was overexpressed inArabidopsis thaliana, the accumulation of flavonoids was improved. In addition, when the wild‐type andEkFLSoverexpressed Arabidopsis plants were treated with ABA and MeJA, compared with WT Arabidopsis,EkFLSoverexpressed Arabidopsis promoted stomatal aperture to influence photosynthesis of the plants, which in turn can promote stress resistance. Meanwhile, under MeJA, NaCl, and PEG treatment,EkFLSoverexpressed in Arabidopsis induced higher accumulation of flavonoids, which significantly enhanced peroxidase (POD) and superoxide dismutase (SOD) activities that can scavenge reactive oxygen species in cells to protect the plant. These results indicated thatEkFLSoverexpression is strongly correlated to the increase of flavonoid synthesis and therefore the tolerance to abiotic stresses in plants, providing a theoretical basis for further improving the quality of medicinal plants and their resistance to abiotic stresses simultaneously.