Controlled Release Strategies for the Combination of Fresh and Lyophilized Platelet-Rich Fibrin on Bone Tissue Regeneration
Controlled Release Strategies for the Combination of Fresh and Lyophilized Platelet-Rich Fibrin on Bone Tissue Regeneration
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新鲜和冻干富含血小板纤维蛋白组合对骨组织再生的控释策略
DOI：
10.1155/2019/4923767
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发表时间：
2019-01-01
影响因子：
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通讯作者：
Zhang, Bin
中科院分区：
文献类型：
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作者：
Liu, Zhongshuang;Jin, Han;Zhang, Bin
本研究旨在探讨新鲜富血小板纤维蛋白（F-PRF）和冻干纤维蛋白（L-PRF）以不同比例联合使用促进骨组织再生的生长因子释放动力学。首先，我们在体外量化了转化生长因子-β1 （TGF-β1）、血管内皮生长因子（VEGF）和血小板衍生生长因子- ab （PDGF-AB）的水平，并分析了它们在不同重量比（F:L=1:1, 1:3, 1:5）下从F-PRF、L-PRF和新鲜/冻干PRF中的释放动力学。第二个实验阶段是研究骨间充质干细胞（BMSCs）的增殖和分化作为对释放因子的功能反应。为了进一步测试其体内成骨潜能，我们将不同的支架（F- prf，或L- prf，或F:L=1:1）植入兔颅骨骨缺损。与阴性对照组相比，在第14天收集的含有不同PRF分泌物的培养基中，BMSCs的增殖和分化均有统计学意义的增加。结果表明，在6周和12周时，新鲜/冻干PRF（1:1）的新骨形成量均明显高于其他缺陷组。我们的数据表明，生长因子浓度和释放动力学是新鲜和冻干PRF结合的结果，这是促进骨再生的有效途径。
The aim of the present study was to investigate growth factors release kinetics for the combination of fresh platelet-rich fibrin (F-PRF) and lyophilized PRF (L-PRF) with different ratios to promote bone tissue regeneration. First, we quantified the level of transforming growth factor-β1 (TGF-β1), vascular endothelial growth factor (VEGF), and platelet-derived growth factor-AB (PDGF-AB) in vitro and analyzed their release kinetics from F-PRF, L-PRF, and the fresh/lyophilized PRF in different weight ratios (F:L=1:1, 1:3, 1:5). The second experimental phase was to investigate the proliferation and differentiation of bone mesenchymal stem cells (BMSCs) as a functional response to the factors released. To further test the osteogenic potential in vivo, different scaffolds (F-PRF, or L-PRF, or F:L=1:1) were implanted in rabbit cranial bone defects. There was a statistically significant increase in proliferation and differentiation of BMSCs when the culture medium contained different PRF exudates collected at day 14 compared with the negative control group. The results showed that the new bone formation in the fresh/lyophilized PRF (1:1) was much more than that of other groups in defects at both 6 and 12 weeks. Our data suggested growth factor concentration and release kinetics as a consequence of fresh and lyophilized PRF combination, which is an effective way for promoting bone regeneration.