Single-Cell Migration as Studied by Scanning Electrochemical Microscopy.
Single-Cell Migration as Studied by Scanning Electrochemical Microscopy.
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DOI：
10.1039/c5ay01944c
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发表时间：
2015-10-21
期刊：
影响因子：
--
通讯作者：
Koley D
中科院分区：
文献类型：
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作者：
Ummadi JG;Joshi VS;Gupta PR;Indra AK;Koley D
用扫描电化学显微镜（SECM）研究单个活头颈癌细胞（SCC 25）的迁移。新开发的石墨糊超微电极（UME）与10 μm Pt-UME相比，显示出显著更少的污染，因此可以用于监测和跟踪单个细胞的迁移模式。我们还使用SECM探针扫描曲线测量细胞迁移期间单个活癌细胞的形态（高度和直径），并确定这些尺寸分别为11 ± 4 μm和40 ± 10 μm。迁移研究显示，同一细胞系内的细胞具有异质迁移模式（迁移和静止），估计迁移速度为8 ± 3 μm/h。然而，同一系的血清饥饿同步化细胞被发现具有非异质性细胞迁移模式，其速度为9 ± 3 μm/h。因此，这种基于SECM的非侵入性技术可能扩展到其他细胞系，以研究细胞生物力学，从而提高对单细胞水平上结构-功能关系的理解。基于SECM的分析方法，用于研究单细胞生物力学，据报道可区分迁移和静止的癌细胞。
Scanning electrochemical microscopy (SECM) was used to study the migration of single live head and neck cancer cells (SCC25). The newly developed graphite paste ultramicroelectrode (UME) showed significantly less fouling in comparison to a 10 μm Pt-UME and thus could be used to monitor and track the migration pattern of a single cell. We also used SECM probe scan curves to measure the morphology (height and diameter) of a single live cancer cell during cellular migration and determined these dimensions to be 11 ± 4 μm and 40 ± 10 μm, respectively. The migration study revealed that cells within the same cell line had a heterogeneous migration pattern (migration and stationary) with an estimated migration speed of 8 ± 3 μm/h. However, serum-starved synchronized cells of the same line were found to have a non-heterogeneous cellular migration pattern with a speed of 9 ± 3 μm/h. Thus, this non-invasive SECM-based technique could potentially be expanded to other cell lines to study cellular biomechanics for improved understanding of the structure-function relationship at the level of a single cell. SECM based analytical methods to study single-cell biomechanics and is reported to differentiate between migrating and stationary cancer cells.