The alpha-keto acids of branched-chain amino acids: simplified derivatization for physiological samples and complete separation as quinoxalinols by packed column gas chromatography.
The alpha-keto acids of branched-chain amino acids: simplified derivatization for physiological samples and complete separation as quinoxalinols by packed column gas chromatography.
复制标题
支链氨基酸的 α-酮酸：简化生理样品的衍生化，并通过填充柱气相色谱法完全分离为喹喔啉醇。
DOI：
10.1016/0003-2697(80)90600-4
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发表时间：
1980
影响因子：
2.9
通讯作者：
Bier,DM
中科院分区：
文献类型：
--
作者：
Schwarz,HP;Karl,IE;Bier,DM
采用一种新型混合固定相，在填充柱气相色谱中，10 min内可将支链α-酮酸类化合物完全分离。生物样品中小于5 nmol的α-酮酸的精确定量是可能的。在少量含水样品中，α-酮酸直接衍生化，无需事先纯化。血浆在衍生化前只需用高氯酸盐脱蛋白并中和。三种主要支链α-酮酸测定的平均相对精密度为± 5.8%。
By use of a relatively new mixed stationary phase, complete separation of the branched-chain α-keto acids as O-trimethylsilyl-quinoxalinol derivatives is achieved within 10 min by packed column gas chromatography. Precise quantification of less than 5 nmol of α-keto acids in biological samples is possible. In small aqueous samples the α-keto acids are directly derivatized without prior purification. Plasma need only be deproteinized by perchlorate and neutralized before derivatization. Average relative precision for determination of the three main branched-chain α-keto acids is ± 5.8%.