Vectors for Efficient and High-Throughput Construction of Fluorescent Drosophila Reporters Using the PhiC31 Site-Specific Integration System
Vectors for Efficient and High-Throughput Construction of Fluorescent Drosophila Reporters Using the PhiC31 Site-Specific Integration System
复制标题
DOI：
10.1002/dvg.20637
复制
发表时间：
2010-07-01
期刊：
影响因子：
1.5
通讯作者：
Lohmann, Ingrid
中科院分区：
文献类型：
--
作者：
Boy, Aurelia L.;Zhai, Zongzhao;Lohmann, Ingrid
果蝇是研究顺式调控元件或增强子转录控制的主要模型系统。在这里，我们提出了一个快速高效的系统，用于大规模分析增强子元件，位点特异性整合到果蝇基因组中。该系统可用于小型项目或高通量方法，利用Gateway克隆技术和PhiC31位点特异性集成系统，允许在预定的基因组位置插入构建体。因此，该系统不仅可以快速简便地分析活体动物中的报告基因表达，还可以通过在同一动物中重组融合到不同荧光蛋白的不同增强子元件，在细胞分辨率上同时分析不同的调控输出。创世纪48:45 -456,2010。(C) 2010 Wiley-Liss, Inc。
The fruit fly Drosophila is a leading model system for the study of transcriptional control by cis-regulatory elements or enhancers. Here, we present a rapid and highly efficient system for the large-scale analysis of enhancer elements, site-specifically integrated into the Drosophila genome. This system, which is scalable for either small projects or high-throughput approaches, makes use of the Gateway cloning technology and the PhiC31 site-specific integration system, which allows the insertion of constructs at predetermined genomic locations. Thus, this system allows not only a fast and easy analysis of reporter gene expression in live animals, but also the simultaneous analysis of different regulatory outputs on a cellular resolution by recombining in the same animal distinct enhancer elements fused to different fluorescent proteins. genesis 48:452-456, 2010. (C) 2010 Wiley-Liss, Inc.