Sensitive fluorescent probes for determination of hydrogen peroxide and glucose based on enzyme-immobilized magnetite/silica nanoparticles
Sensitive fluorescent probes for determination of hydrogen peroxide and glucose based on enzyme-immobilized magnetite/silica nanoparticles
复制标题
基于酶固定磁铁矿/二氧化硅纳米粒子的用于测定过氧化氢和葡萄糖的灵敏荧光探针
DOI：
10.1007/s00216-009-3118-9
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发表时间：
2009-12-01
影响因子：
4.3
通讯作者：
Tang, Heqing
中科院分区：
文献类型：
--
作者：
Chang, Qing;Zhu, Lihua;Tang, Heqing
在戊二醛存在下，将辣根过氧化物酶（HRP）固定在Fe3O4/SiO2磁性核壳纳米颗粒上，建立了检测过氧化氢和葡萄糖的灵敏荧光探针。此外，固定化酶具有良好的催化活性，通过磁分离可以很容易地完全回收，并且回收的酶固定化Fe3O4/SiO2纳米颗粒可以作为催化剂重复使用而不会失活。酶标纳米颗粒能够激活过氧化氢（H2O2），过氧化氢将非荧光3-（4-羟基苯基）丙酸氧化为荧光产物，其最大发射波长为409 nm。在优化条件下，在H2O2浓度范围为5.0 × 10(-9) ~ 1.0 × 10(-5) mol L-1范围内建立了线性校准曲线，检出限为2.1 × 10(-9) mol L-1。利用葡萄糖氧化酶和酶固定化Fe3O4/SiO2纳米颗粒，建立了一种灵敏、选择性的葡萄糖检测方法。产物的荧光强度与葡萄糖浓度在5.0 × 10(-8) ~ 5.0 × 10(-5) mol L-1范围内线性响应良好，检出限为1.8 × 10(-8) mol L-1。该方法成功地应用于人血清中葡萄糖的测定。
Sensitive fluorescent probes for the determination of hydrogen peroxide and glucose were developed by immobilizing enzyme horseradish peroxidase (HRP) on Fe3O4/SiO2 magnetic core-shell nanoparticles in the presence of glutaraldehyde. Besides its excellent catalytic activity, the immobilized enzyme could be easily and completely recovered by a magnetic separation, and the recovered HRP-immobilized Fe3O4/SiO2 nanoparticles were able to be used repeatedly as catalysts without deactivation. The HRP-immobilized nanoparticles were able to activate hydrogen peroxide (H2O2), which oxidized non-fluorescent 3-(4-hydroxyphenyl)propionic acid to a fluorescent product with an emission maximum at 409 nm. Under optimized conditions, a linear calibration curve was obtained over the H2O2 concentrations ranging from 5.0 x 10(-9) to 1.0 x 10(-5) mol L-1, with a detection limit of 2.1 x 10(-9) mol L-1. By simultaneously using glucose oxidase and HRP-immobilized Fe3O4/SiO2 nanoparticles, a sensitive and selective analytical method for the glucose detection was established. The fluorescence intensity of the product responded well linearly to glucose concentration in the range from 5.0 x 10(-8) to 5.0 x 10(-5) mol L-1 with a detection limit of 1.8 x 10(-8) mol L-1. The proposed method was successfully applied for the determination of glucose in human serum sample.