QUANTITATIVE STUDY OF MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1) IN CEREBROSPINAL-FLUID AND CYST FLUID FROM PATIENTS WITH MALIGNANT GLIOMA
QUANTITATIVE STUDY OF MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1) IN CEREBROSPINAL-FLUID AND CYST FLUID FROM PATIENTS WITH MALIGNANT GLIOMA
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DOI：
10.1093/jnci/85.22.1836
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发表时间：
1993-11-17
期刊：
影响因子：
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通讯作者：
USHIO, Y
中科院分区：
文献类型：
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作者：
KURATSU, J;YOSHIZATO, K;USHIO, Y
背景：单核细胞趋化蛋白-1（MCP-1）是一种由76个氨基酸组成的蛋白质，具有吸引单核细胞的作用。体外研究已经报道了恶性胶质瘤细胞中MCP-1信使RNA的高水平表达以及MCP-1的存在。目的：我们的目的是确定MCP-1检测是否可用于临床：1）区分恶性胶质瘤和良性胶质瘤以及中枢神经系统的非肿瘤性疾病; 2）检测胶质瘤细胞的蛛网膜下腔播散。研究方法：用我们以前开发的夹心酶联免疫吸附试验（ELISA）测定脑脊液（CSF）和囊液中的MCP-1水平。我们测量了19例恶性胶质瘤患者（胶质母细胞瘤，10例;间变性星形细胞瘤，6例;间变性少突胶质细胞瘤，2例;室管膜母细胞瘤，1例），9例良性胶质瘤患者和7例中枢神经系统非肿瘤疾病患者的CSF样本中MCP-1水平。还对4例恶性胶质瘤（间变性星形细胞瘤）患者的囊液进行了检测。还研究了CSF中MCP-1浓度与恶性胶质瘤细胞蛛网膜下腔播散的相关性。结果如下：恶性胶质瘤患者脑脊液中MCP-1浓度（平均值± SE）为2.3 ± 0.4 ng/mL，明显高于良性胶质瘤患者（0.6 ± 0.1 ng/mL）（P
Background: Monocyte chemoattractant protein-1 (MCP-1) is a 76-amino acid protein that attracts monocytes. In vitro studies have reported high levels of MCP-1 messenger RNA expression, as well as the presence of MCP-1, in malignant glioma cells. Purpose: Our purpose was to determine whether an MCP-1 assay could be used in a clinical setting 1) to differentiate malignant from benign gliomas and from nontumor disorders of the central nervous system and 2) to detect subarachnoid dissemination of glioma cells. Methods: MCP-1 levels in cerebrospinal fluid (CSF) and cyst fluid were measured with a sandwich enzyme-linked immunosorbent assay (ELISA) that we had previously developed. We measured MCP-1 levels in CSF samples from 19 patients with malignant glioma (glioblastoma, 10; anaplastic astrocytoma, six; anaplastic oligodendroglioma, two; and ependymoblastoma, one), nine patients with benign glioma, and seven patients with nontumor disorders of the central nervous system. Cyst fluids from four patients with malignant glioma (anaplastic astrocytoma) were also tested. The correlation between MCP-1 concentration in the CSF and subarachnoid dissemination of malignant glioma cells was also studied. Results: The MCP-1 concentration (mean +/- SE) in CSF samples from patients with malignant glioma (2.3 +/- 0.4 ng/mL) was significantly higher than that from patients with benign glioma (0.6 +/- 0.1 ng/mL) (P