The Caenorhabditis elegans p120 catenin homologue, JAC-1, modulates cadherin-catenin function during epidermal morphogenesis.
The Caenorhabditis elegans p120 catenin homologue, JAC-1, modulates cadherin-catenin function during epidermal morphogenesis.
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秀丽隐杆线虫P120 Catenin同源物，JAC-1，在表皮形态发生过程中调节钙粘蛋白 - 钙蛋白的功能。
DOI：
10.1083/jcb.200212136
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发表时间：
2003-07-07
影响因子：
7.8
通讯作者：
Hardin, Jeff
中科院分区：
文献类型：
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作者：
Pettitt, Jonathan;Cox, Elisabeth A;Broadbent, Ian D;Flett, Aileen;Hardin, Jeff
钙粘蛋白-连环蛋白复合体是动物发育过程中组织形态发生所必需的。在培养的哺乳动物细胞中，p120连环蛋白（p120 ctn）是钙粘蛋白-连环蛋白复合体功能的重要调节因子。然而，关于p120 ctn家族成员在体内钙粘蛋白依赖性事件中的作用的信息是有限的。我们研究了单一秀丽隐杆线虫p120 ctn同源物JAC-1（胞膜结构域[JMD]相关连环蛋白）在表皮形态发生过程中的作用。与其他p120 ctn家族成员类似，JAC-1结合经典钙粘蛋白HMR-1的JMD，并且GFP标记的JAC-1以HMR-1依赖性方式定位于粘附连接。令人惊讶的是，使用RNA干扰（RNAi）消除JAC-1表达不会导致胚胎或胚后发育中的任何明显缺陷。然而，jac-1（RNAi）确实增加了由hmp-1/α-catenin基因中的亚型突变引起的形态发生缺陷的严重性和易变性。在这些hmp-1突变体中，jac-1缺失导致胚胎不能伸长成蠕虫状，这一过程涉及表皮收缩。与失败的延伸是脱离肌动蛋白束从表皮粘附连接和失败，以维持在粘附连接钙粘蛋白。这些结果表明，JAC-1在C.优雅
The cadherin–catenin complex is essential for tissue morphogenesis during animal development. In cultured mammalian cells, p120 catenin (p120ctn) is an important regulator of cadherin–catenin complex function. However, information on the role of p120ctn family members in cadherin-dependent events in vivo is limited. We have examined the role of the single Caenorhabditis elegans p120ctn homologue JAC-1 (juxtamembrane domain [JMD]–associated catenin) during epidermal morphogenesis. Similar to other p120ctn family members, JAC-1 binds the JMD of the classical cadherin HMR-1, and GFP-tagged JAC-1 localizes to adherens junctions in an HMR-1–dependent manner. Surprisingly, depleting JAC-1 expression using RNA interference (RNAi) does not result in any obvious defects in embryonic or postembryonic development. However, jac-1(RNAi) does increase the severity and penetrance of morphogenetic defects caused by a hypomorphic mutation in the hmp-1/α-catenin gene. In these hmp-1 mutants, jac-1 depletion causes failure of the embryo to elongate into a worm-like shape, a process that involves contraction of the epidermis. Associated with failed elongation is the detachment of actin bundles from epidermal adherens junctions and failure to maintain cadherin in adherens junctions. These results suggest that JAC-1 acts as a positive modulator of cadherin function in C. elegans.