Development of the smooth muscle foam cell: uptake of macrophage lipid inclusions.
Development of the smooth muscle foam cell: uptake of macrophage lipid inclusions.
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DOI：
10.1073/pnas.83.20.7760
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发表时间：
1986-10
影响因子：
11.1
通讯作者：
G. Wolfbauer;J. M. Glick;L. Minor;G. Rothblat
中科院分区：
文献类型：
--
作者：
G. Wolfbauer;J. M. Glick;L. Minor;G. Rothblat
探讨了动脉粥样硬化病变中平滑肌泡沫细胞形成的可能机制。培养的巨噬细胞(J774细胞系)在高胆固醇磷脂分散体存在的情况下，暴露于乙酰化低密度脂蛋白，诱导其形成细胞质胆固醇酯包涵体。用短时间超声破碎巨噬细胞，浮选分离包涵体。当这些包涵体与培养的平滑肌细胞直接接触时，观察到细胞以时间和剂量依赖的方式摄取包涵体。光镜和电子显微镜下可见细胞胞浆内有脂包涵体存在。几种代谢抑制剂抑制了血管平滑肌细胞对包涵体脂质的摄取，表明这一过程依赖于代谢活性。观察到胆固醇酯适度但显著的水解，表明储存的胆固醇酯在代谢中是可用的。
A possible mechanism for the formation of smooth muscle foam cells in the atherosclerotic lesion was explored. Cultured macrophages (J774 cell line) were induced to form cytoplasmic cholesteryl ester inclusions by exposure to acetylated low density lipoprotein in the presence of cholesterol-rich phospholipid dispersions. The macrophages were disrupted by brief sonication, and the inclusions were isolated by flotation. When these inclusions were placed in direct contact with cultured smooth muscle cells, cellular uptake of the inclusions in a time- and dose-dependent manner was observed. Light and electron microscopy indicated the presence of lipid inclusions throughout the cytoplasm of the cells. Uptake of inclusion lipid by the smooth muscle cells was inhibited by several metabolic inhibitors, indicating that the process is dependent on metabolic activity. A modest but significant hydrolysis of the cholesteryl ester was observed, showing that the stored cholesteryl esters are metabolically available.