Quantification of immunoglobulin free light chains in cerebrospinal fluid by nephelometry
Quantification of immunoglobulin free light chains in cerebrospinal fluid by nephelometry
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DOI：
10.1007/s10875-005-5371-9
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发表时间：
2005-07-01
影响因子：
9.1
通讯作者：
Boucraut, J
中科院分区：
文献类型：
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作者：
Desplat-Jégo, S;Feuillet, L;Boucraut, J
寡克隆游离轻链（FLC）条带已在多发性硬化（MS）中描述，并应与疾病活动相关。然而，由于方法的差异，报告了研究之间的差异。一种新的定量，快速，自动化的方法，使用浊度法现在可用。我们的目的是调查的利益，这种方法的MS的诊断和预后。为此，FLC指数确定配对样本的CSF和血清连续和连续的患者从同一个神经科。我们招募了89名患者（33名MS，15名“可能MS”和41名对照），并与IgG指数，IgG寡克隆带和临床MS进展标准相关。结果表明：（1）FLC kappa指数对MS的诊断敏感性高于IgG指数，但特异性低于IgG指数;（2）2例MS患者寡克隆带阴性，但Kappa指数阳性;（3）FLC kappa指数与MS临床分期及病情进展无相关性。它的前鼻侧兴趣仍有待于在更大的可能MS患者队列中确定。
Oligoclonal free light chains (FLC) banding has been described in multiple sclerosis (MS) and should be correlated with disease activity. However, discrepancies between studies have been reported because of differences in methods. A new quantitative, rapid, and automated method using nephelometry is now available. Our objective was to investigate the interest of this method for the diagnosis and prognosis of MS. For this purpose, FLC index was determined in paired samples of CSF and serum from consecutive and unselected patients from the same department of neurology. We enrolled 89 patients (33 MS, 15 "possible MS", and 41 controls) and correlated with IgG index, IgG oligoclonal banding, and clinical MS progression criteria. The main results were (1) FLC kappa index was more sensitive but less specific than IgG index for the diagnosis of MS, (2) two MS patients were negative for oligoclonal banding but exhibited a positive kappa index, (3) no relation between FLC kappa indices, MS clinical criteria, and disease progression was found. In conclusion, FLC kappa index should be considered as a useful complementary test for MS diagnosis. Its pronostic interest remains to be determined on a larger cohort of possible MS patients.