HIGH-THROUGHPUT SCREEN FOR DETECTING ANTIMYCOBACTERIAL AGENTS
HIGH-THROUGHPUT SCREEN FOR DETECTING ANTIMYCOBACTERIAL AGENTS
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DOI：
10.1128/aac.39.10.2235
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发表时间：
1995-10-01
影响因子：
4.9
通讯作者：
DUNCAN, K
中科院分区：
文献类型：
--
作者：
CHUNG, GAC;AKTAR, Z;DUNCAN, K
已经开发出一种简单、稳健的检测系统，可用于筛选分枝杆菌生长的抑制剂。使用快速生长的腐生菌金黄色分枝杆菌菌株作为测试生物体。对其生长的抑制可以高度预测抗结核分枝杆菌的活性，但由于其生长特征和高度传染性，结核分枝杆菌本身不能用于筛查。通过测量细胞对放射性标记尿嘧啶的摄取来监测金黄色分枝杆菌在测试样品存在下的活力。在微量滴定板形式中，屏幕每天可以测试数千个样品。
A simple, robust assay system which can be used to screen for inhibitors of mycobacterial growth has been developed. A strain of the rapidly growing saprophyte Mycobacterium aurum is used as the test organism. Inhibition of its growth is highly predictive of activity against Mycobacterium tuberculosis, which cannot itself be used in screening because of its growth characteristics and highly infectious nature. The viability of M. aurum in the presence of a test sample is monitored by measuring the uptake of radiolabelled uracil into the cells. In a microtiter plate format, the screen has the potential for testing several thousand samples per day.