Analytical solutions for time-resolved fluorescence lifetime imaging in a turbid medium such as tissue
Analytical solutions for time-resolved fluorescence lifetime imaging in a turbid medium such as tissue
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DOI：
10.1364/josaa.18.001523
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发表时间：
2001-07-01
影响因子：
1.9
通讯作者：
Gandjbakhche, A
中科院分区：
文献类型：
--
作者：
Hattery, D;Chernomordik, V;Gandjbakhche, A
一个分析解决方案的开发，以量化特定的位点荧光团的寿命扰动发生，例如，当局部代谢状态是从周围组织不同。当荧光团分布在整个高度混浊的介质中，并且感兴趣的部位嵌入距离源和检测器许多平均散射距离时，可以使用该解决方案。利用随机游走理论将寿命扰动与光子渡越延迟区分开来。该解析解需要在激发和发射波长处的组织散射和吸收特性的先验知识，所述先验知识可以从并发时间分辨反射测量获得。此外，该解决方案已与精确的，数值求解的解决方案。因此，所提出的解决方案形成了在诸如组织的混浊介质中的实际寿命成像的基础。(C)2001年美国光学学会。
An analytical solution is developed to quantify a site-specific fluorophore lifetime perturbation that occurs, for example, when the local metabolic status is different from that of surrounding tissue. This solution may be used when fluorophores are distributed throughout a highly turbid media and the site of interest is embedded many mean scattering distances from the source and the detector. The perturbation in lifetime is differentiated from photon transit delays by random walk: theory. This analytical solution requires a priori knowledge of the tissue-scattering and absorption properties at the excitation and emission wavelengths that may be obtained from concurrent time-resolved reflection measurements. Additionally, the solution has been compared with the exact, numerically solved solution. Thus the presented solution forms the basis for practical lifetime imaging in turbid media such as tissue. (C) 2001 Optical Society of America.