PpV, acting via the JNK pathway, represses apoptosis during normal development of Drosophila wing
PpV, acting via the JNK pathway, represses apoptosis during normal development of Drosophila wing
复制标题
PpV 通过 JNK 途径发挥作用，抑制果蝇翅膀正常发育过程中的细胞凋亡。
DOI：
10.1007/s10495-018-1479-2
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发表时间：
2018-10-01
期刊：
影响因子：
7.2
通讯作者：
Su, Ying
中科院分区：
文献类型：
--
作者：
Chi, Chunli;Wang, Liguo;Su, Ying
细胞凋亡是多细胞生物体发育所必需的基本生物学过程之一。不适当的细胞凋亡调控可导致严重的发育异常和疾病。因此，对细胞凋亡的控制，不仅是对其激活的控制，也是对其的抑制，在发育过程中至关重要。与广泛的诱导凋亡研究相比，其在某些细胞群体中更为重要的抑制机制实际上还远未被很好地了解。在这里，我们报道了一种丝氨酸/苏氨酸蛋白磷酸酶V(PPV)在果蝇翅膀发育过程中对细胞凋亡的抑制作用。我们观察到，RNAi抑制了翅膀成像盘中的PPV，导致了异位细胞死亡和caspase激活，从而导致了成年翅膀的缺陷。此外，击倒PPV还触发了c-jun氨基末端激酶(JNK)信号的激活，这是一种进化上保守的细胞内信号，在许多生物和实验系统中被认为参与了调节细胞凋亡的机制。干扰JNK信号转导足以抑制PPV对翅膀发育的影响。我们共同提供的证据表明，PPV可能通过JNK途径维持凋亡信号的沉默，是正常翅膀发育所必需的。
Apoptosis is one of the main fundamental biological processes required for development of multicellular organisms. Inappropriate regulation of apoptosis can lead to severe developmental abnormalities and diseases. Therefore, the control of apoptosis, not only for its activation but also for its inhibition, is critically important during development. In contrast to the extensive studies of apoptosis induction, its inhibitory mechanisms that are even more vital in certain populations of cells actually are very far from being well understood. Here we report an inhibitory role of protein phosphatase V (PpV), a serine/threonine protein phosphatase, in controlling the apoptosis during Drosophila wing development. We observed that inhibition of ppv by RNAi in wing imaginal discs induced ectopic cell death and caspase activation, thus, resulted in a defective adult wing. Moreover, knocking-down ppv triggered the activation of c-Jun N-terminal kinase (JNK) signal, an evolutionarily conserved intracellular signaling that has been implicated to modulate the apoptotic machinery in many biological and experimental systems. Disrupting the JNK signal transduction was adequate to suppress the ppv effects for wing development. Together, we provided the evidence to demonstrate that ppv is required for normal wing development in maintaining the silence of apoptotic signal possibly through JNK pathway.