THE CARBOXYL EXTENSIONS OF 2 RAT UBIQUITIN FUSION PROTEINS ARE RIBOSOMAL-PROTEINS S27A AND L40
THE CARBOXYL EXTENSIONS OF 2 RAT UBIQUITIN FUSION PROTEINS ARE RIBOSOMAL-PROTEINS S27A AND L40
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DOI：
10.1006/bbrc.1995.2518
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发表时间：
1995-10-13
影响因子：
3.1
通讯作者：
WOOL, IG
中科院分区：
文献类型：
--
作者：
CHAN, YL;SUZUKI, K;WOOL, IG
对两种大鼠重组cdna进行了表征；它们编码的融合蛋白在NH2端具有保守的76个氨基酸泛素，在羧基端具有延伸核糖体蛋白S27a （SO个氨基酸，分子量为9,397）或L40（52个氨基酸，分子量为6,177）。融合蛋白在网状细胞裂解物中被加工成泛素和S27a或L40。DNA与核DNA的杂交表明，S27a基因有14到19个拷贝，L40基因有6到10个拷贝。泛素- s27a的mRNA大约有700个核苷酸，泛素- l40大约有650个核苷酸。核糖体蛋白S27a和L40含有C-2-C-2品种的锌指基序。S27a和L40与其他物种的核糖体蛋白有关。(C) 1995学术出版社，Inc。
Two rat recombinant cDNAs were characterized; they encode fusion proteins that have at their NH2 terminus the conserved 76 amino acid ubiquitin and at their carboxyl terminus the extension ribosomal proteins S27a (SO amino acids and a molecular weight of 9,397) or L40 (52 amino acids and a molecular weight of 6,177). The fusion proteins are processed in a reticulocyte lysate to ubiquitin and either S27a or L40. Hybridization of cDNAs to digests of nuclear DNA suggests that there are 14 to 19 copies of the S27a, and 6 to 10 of the L40, genes. The mRNA for ubiquitin-S27a has about 700 nucleotides and ubiquitin-L40 about 650. Ribosomal proteins S27a and L40 contain zinc finger motifs of the C-2-C-2 variety. S27a and L40 are related to ribosomal proteins from other species. (C) 1995 Academic Press, Inc.