Haemophilus ducreyi is susceptible to protegrin
Haemophilus ducreyi is susceptible to protegrin
复制标题
DOI：
10.1128/aac.42.10.2690
复制
发表时间：
1998-10-01
影响因子：
4.9
通讯作者：
Spinola, SM
中科院分区：
文献类型：
--
作者：
Fortney, K;Totten, PA;Spinola, SM
Protegrin 是在猪白细胞中发现的强效抗菌肽，具有对抗性传播病原体淋病奈瑟氏菌、沙眼衣原体和 I 型人类免疫缺陷病毒的活性。我们测试了合成的 protegrin 1 (PG-I) 对九种杜克雷嗜血杆菌分离株的活性，杜克雷嗜血杆菌是淋病的病原体。 软下疳，测试微生物包括 CIP 542（模式菌株）。 35000HP（35000 的人类传代变体）、35000HP-RSM2 tan 同基因 D-甘油-D-甘露庚糖基转移酶突变体（35000HP 的突变体）和 6 个临床分离株。这些分离株在流行病学上不相关，代表三种 HindIII 核糖核酸型，并且具有不同的抗菌药物耐药性模式。在杀菌试验中，活分离株的杀菌速度很快；杀死了bq合成PG-1，在径向扩散测定中，所有九个分离株都对PG-I极其敏感，这些数据突出了protegrin作为局部制剂开发以预防许多性传播疾病（包括软下疳）的潜力。
Protegrins, potent antimicrobial peptides found in pol cine leukocytes, have activity against the sexually transmitted pathogens Neisseria gonorrhoeae, Chlamydia trachomatis, and human immunodeficiency virus type I. We tested synthetic protegrin 1 (PG-I) for activity against nine isolates of Haemophilus ducreyi, the etiologic agent of chancroid, The test organisms included CIP 542 (the type strain). 35000HP (a human-passaged variant of 35000), 35000HP-RSM2 tan isogenic D-glycero-D-manno-heptosyltransferase mutant of 35000HP), and six clinical isolates. The isolates were epidemiologically unrelated, represented three HindIII ribotypes, and had varying antimicrobial resistance patterns. In bactericidal assays, live isolates were rapidly; killed bq synthetic PG-1, In radial diffusion assays, all nine isolates were exquisitely sensitive to PG-I, These data highlight the potential of protegrins for development as topical agents to prevent many sexually transmitted diseases, including chancroid.