Myo-inositol-1-phosphate synthase (Ino-1) functions as a protection mechanism in Corynebacterium glutamicum under oxidative stress.
Myo-inositol-1-phosphate synthase (Ino-1) functions as a protection mechanism in Corynebacterium glutamicum under oxidative stress.
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肌醇-1-磷酸合酶 (Ino-1) 在氧化应激下作为谷氨酸棒杆菌的保护机制
DOI：
10.1002/mbo3.721
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发表时间：
2019-05
期刊：
影响因子：
3.4
通讯作者：
Si M
中科院分区：
文献类型：
--
作者：
Chen C;Chen K;Su T;Zhang B;Li G;Pan J;Si M
在有氧代谢和氧化应激中产生的活性氧（ROS）导致大分子损伤，例如对蛋白质、脂质和DNA的损伤，其可以通过氧化还原缓冲剂真菌硫醇（AcCys-GlcN-Ins，MSH）消除。肌肌醇磷酸合酶（Ino-1）催化MSH合成的第一个关键步骤，因此在生物体的生长中起着关键作用。虽然Ino-1在真核生物中已经得到了系统的研究，但它们在细菌中的生理和生化功能仍然很大程度上未知。在这项研究中，我们报告Ino-1在革兰氏阳性放线菌谷氨酸棒杆菌的氧化应激抗性中起着重要作用。在各种应激条件下，ino-1基因的缺失导致细胞活力降低、ROS产生增加和蛋白质羰基化水平恶化。Ino-1在抗氧化应激中的生理作用通过Δino-1突变体中MSH的缺失得到证实。此外，我们发现Ino-1在谷氨酸棒杆菌中的同源表达产生了功能活性蛋白，而在大肠杆菌BL 21（DE 3）中表达时，它缺乏可测量的活性。对分子量（Mr）的检查表明，在大肠杆菌BL 21（DE 3）中表达的Ino-1未折叠成催化活性构象。总之，结果明确表明Ino-1对于谷氨酸棒杆菌介导氧化抗性很重要。
Reactive oxygen species (ROS) generated in aerobic metabolism and oxidative stress lead to macromolecules damage, such as to proteins, lipids, and DNA, which can be eliminated by the redox buffer mycothiol (AcCys‐GlcN‐Ins, MSH). Myo‐inositol‐phosphate synthase (Ino‐1) catalyzes the first committed step in the synthesis of MSH, thus playing a critical role in the growth of the organism. Although Ino‐1s have been systematically studied in eukaryotes, their physiological and biochemical functions remain largely unknown in bacteria. In this study, we report that Ino‐1 plays an important role in oxidative stress resistance in the gram‐positive Actinobacteria Corynebacterium glutamicum. Deletion of the ino‐1 gene resulted in a decrease in cell viability, an increase in ROS production, and the aggravation of protein carbonylation levels under various stress conditions. The physiological roles of Ino‐1 in the resistance to oxidative stresses were corroborated by the absence of MSH in the Δino‐1 mutant. In addition, we found that the homologous expression of Ino‐1 in C. glutamicum yielded a functionally active protein, while when expressed in Escherichia coli BL21(DE3), it lacked measurable activity. An examination of the molecular mass (Mr) suggested that Ino‐1 expressed in E. coli BL21(DE3) was not folded in a catalytically competent conformation. Together, the results unequivocally showed that Ino‐1 was important for the mediation of oxidative resistance by C. glutamicum.
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影响因子：
9.8
作者：
Hondorp ER;Matthews RG
通讯作者：
Matthews RG
影响因子：
64.5
作者：
Kohanski, Michael A.;Dwyer, Daniel J.;Collins, James J.
通讯作者：
Collins, James J.
DOI：
10.1111/j.1432-1033.1985.tb08997.x
发表时间：
1985-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
EGGELING, L;SAHM, H
通讯作者：
SAHM, H
影响因子：
3.6
作者：
Movahedzadeh, F;Smith, DA;Stoker, NG
通讯作者：
Stoker, NG
影响因子：
4.8
作者：
Newton, Gerald L.;Ta, Philong;Fahey, Robert C.
通讯作者：
Fahey, Robert C.