Pitx1 and Pitx2 are required for development of hindlimb buds
Pitx1 and Pitx2 are required for development of hindlimb buds
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DOI：
10.1242/dev.00192
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发表时间：
2003-01-01
期刊：
影响因子：
4.6
通讯作者：
Drouin, J
中科院分区：
文献类型：
--
作者：
Marcil, A;Dumontier, É;Drouin, J
两个密切相关的同源异型盒转录因子，Pitx1和Pitx2，已被牵连在侧板中胚层衍生物的图案化：Pitx1的规格后肢身份和Pitx2的测定偏侧。我们发现，一起，Pitx1和Pitx2需要形成后肢芽，当存在于有限的剂量，近端（股骨）和前（胫骨和数字1）后肢结构的发展。虽然Pitx1在整个发育的后肢芽中表达，但Pitx2在肢芽间充质本身中不表达，而是在芽生长之前与Pitx1在假定的后肢领域中共表达。因此，Pitx1和Pitx2基因是持续的后肢芽生长和后肢形成所必需的。
Two closely related homeobox transcription factors, Pitx1 and Pitx2, have been implicated in patterning of lateral plate mesoderm derivatives: Pitx1 for specification of hindlimb identity and Pitx2 for determination of laterality. We show that, together, Pitx1 and Pitx2 are required for formation of hindlimb buds and, when present in limited doses, for development of proximal (femur) and anterior (tibia and digit 1) hindlimb structures. Although Pitx1 is expressed throughout developing hindlimb buds, Pitx2 is not expressed in limb bud mesenchyme itself, but is co-expressed with Pitx1 in the presumptive hindlimb field before bud growth. Thus, Pitx1 and Pitx2 genes are required for sustained hindlimb bud growth and formation of hindlimbs.