A biocompatible condensation reaction for the labeling of terminal cysteine residues on proteins.
A biocompatible condensation reaction for the labeling of terminal cysteine residues on proteins.
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DOI：
10.1002/anie.200903627
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Rao J
中科院分区：
文献类型：
--
作者：
Ren H;Xiao F;Zhan K;Kim YP;Xie H;Xia Z;Rao J
该通信描述了一种新的蛋白质标记方法，该方法使用单个氨基酸标签-N-末端半胱氨酸残基-和携带氰基苯并噻唑单元的小分子探针，用于体外和活细胞表面的蛋白质特异性标记。这种简单的连接反应在生理条件下具有高度的特异性，并且应该为目前可用的蛋白质标记方法提供重要的替代方案。
This communication describes a novel protein labeling method that uses a single amino-acid tag — N-terminal cysteine residue — and small-molecule probes carrying the cyanobenzothiazole unit for specific labeling of proteins in vitro and at the surface of live cells. This simple ligation reaction proceeds with a high degree of specificity in physiological conditions, and should offer an important alternative to currently available protein labeling methods.