Elevation of macrophage-derived chemokine in eosinophilic pneumonia : a role of alveolar macrophages
Elevation of macrophage-derived chemokine in eosinophilic pneumonia : a role of alveolar macrophages
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DOI：
10.2152/jmi.52.85
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发表时间：
2005-02-01
影响因子：
0.7
通讯作者：
Sone, Saburo
中科院分区：
文献类型：
--
作者：
Manabe, Kazuyoshi;Nishioka, Yasuhiko;Sone, Saburo
巨噬细胞源性趋化因子（MDC/CCL 22）和胸腺活化调节趋化因子（TARC/CCL 17）是CC趋化因子受体4的配体。最近，TARC已被报道在特发性嗜酸性粒细胞性肺炎（IEP）的发病机制中发挥作用。本研究的目的是评估MDC在IEP和其他间质性肺病（ILD）中的作用。采用酶联免疫吸附法测定ILD患者和健康志愿者支气管肺泡灌洗液（BALF）中MDC和TARC的含量。我们还通过实时定量逆转录-聚合酶链反应检测了肺泡巨噬细胞（AM）中MDC mRNA的表达。MDC和TARC仅在IEP患者的BALF中检测到。MDC浓度均高于TARC。IEP和TARC的MDC水平具有相关性。IEP患者AM中MDC的表达量在蛋白和mRNA水平均显著高于HV。当患者达到缓解时，IEP BALF中的MDC显著降低。结果提示，MDC可能参与了IEP的发病过程，而AM可能参与了IEP中MDC的升高。
Macrophage-derived chemokine (MDC/CCL22) andthymus-andactivation-regulated chemokine (TARC/CCL17) are ligands for CC chemokine receptor 4. Recently, TARC has been reported to play a role in the pathogenesis of idiopathic eosinophilic pneumonia (IEP). The purpose of this study was to evaluate the role of MDC in IEP and other interstitial lung diseases (ILDs). MDC and TARC in the bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay in patients with ILDs and healthy volunteers (HV). We also examined the expression of MDC mRNA in alveolar macrophages (AM) by real-time quantitative reverse transcriptase-polymerase chain reaction. Both MDC and TARC were detected only in BALF obtained from IEP patients. The concentration of MDC was higher than that of TARC in all cases. The level of MDC in IEP correlated with that of TARC. AM from IEP patients expressed a significantly higher amount of MDC than that from HV at the levels of protein and mRNA. MDC in BALF from IEP dramatically decreased when patients achieved remission. These findings suggest that MDC, in addition to TARC, might be involved in the pathogenesis of IEP, and AM play a role in the elevation of MDC in IEP.