Two nitridergic peptides are encoded by the gene capability in Drosophila melanogaster
Two nitridergic peptides are encoded by the gene capability in Drosophila melanogaster
复制标题
DOI：
10.1152/ajpregu.00584.2001
复制
发表时间：
2002-05-01
影响因子：
2.8
通讯作者：
Dow, JAT
中科院分区：
文献类型：
--
作者：
Kean, L;Cazenave, W;Dow, JAT
果蝇基因（capability，capa）位于染色体3R上99 D处，可能编码三种神经肽：GANMGLYAFPRV-酰胺（capa-1）、ASGLVAFPRV-酰胺（capa-2）和TGPSASSGLWGPRL-酰胺（capa-3）。Capa-1和capa-2与鳞翅目激素心脏加速肽2b相关，而capa-3是信息素生物合成激活神经肽/滞育激素/焦激肽家族的新成员。通过免疫细胞化学，我们确定了四对神经内分泌细胞可能释放capa肽进入血淋巴：一对在食管下神经节和其他三个在腹部神经节。在Malpighian（肾）小管中，capa-1和capa-2增加液体分泌速率，刺激一氧化氮产生，并升高主细胞中的细胞内Ca 2+和cGMP。鸟苷酸环化酶抑制剂亚甲蓝可抑制卡帕刺激的体液分泌，但不抑制细胞内Ca 2+浓度升高。capa-1和capa-2的作用不是协同的，这意味着两者作用于肾小管中的相同途径。因此capa基因是第一个被证明编码通过一氧化氮作用于肾液产生的神经肽的基因。
A Drosophila gene (capability, capa) at 99D on chromosome 3R potentially encodes three neuropeptides: GANMGLYAFPRV-amide (capa-1), ASGLVAFPRV-amide (capa-2), and TGPSASSGLWGPRL-amide (capa-3). Capa-1 and capa-2 are related to the lepidopteran hormone cardioacceleratory peptide 2b, while capa-3 is a novel member of the pheromone biosynthesis-activating neuropeptide/diapause hormone/pyrokinin family. By immunocytochemistry, we identified four pairs of neuroendocrine cells likely to release the capa peptides into the hemolymph: one pair in the subesophageal ganglion and the other three in the abdominal neuromeres. In the Malpighian (renal) tubule, capa-1 and capa-2 increase fluid secretion rates, stimulate nitric oxide production, and elevate intracellular Ca2+ and cGMP in principal cells. Capa-stimulated fluid secretion, but not intracellular Ca2+ concentration rise, is inhibited by the guanylate cyclase inhibitor methylene blue. The actions of capa-1 and capa-2 are not synergistic, implying that both act on the same pathways in tubules. The capa gene is thus the first to be shown to encode neuropeptides that act on renal fluid production through nitric oxide.