Pleiotropic effects of the sdw1 locus in barley populations representing different rounds of recombination
Pleiotropic effects of the sdw1 locus in barley populations representing different rounds of recombination
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DOI：
10.1016/j.ejbt.2014.07.005
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发表时间：
2014-09-01
影响因子：
2.7
通讯作者：
Ćwiek, Hanna
中科院分区：
文献类型：
--
作者：
Kuczyńska, Anetta;Mikołajczak, Krzysztof;Ćwiek, Hanna
背景资料：在本研究中，代表不同轮的重组群体用于分析与sdw 1/denso基因座相关的表型效应。其他研究大多只关注一种类型的人群。在sdw 1/denso位点的同一位置上定位的多个不同的QTL可能表明该基因的多效性或数量性状基因间的紧密连锁。迄今为止，研究结果还没有明确证明这两个phenomenon.Results：育种和分子定位实验进行了检查200单种子血统（SSD）和60个双单倍体（DH）线从Maresi（与半矮秆基因）和Pomo杂交组合。他们进行了评估的类型的少年生长习性和某些农艺性状收获后进行了测量。分析了平均值、标准误和效应显著性的估计值。在分析的特点，最大的变异性，获得了与匍匐生长习性的基因型。利用微卫星标记（SSR）分析了与sdw 1/denso位点的共分离，发现与sdw 1/denso位点连锁最紧密的标记为Bmag 0013、Bmag 0877、Bmag 0306 b。结论：半矮秆基因sdw 1/denso在SSD和DH群体中均对株高、抽穗期和开花期有影响，可能具有多效性。在SSD群体中，sdw 1/denso基因座与穗粒重和千粒重存在部分连锁。(C)2014年瓦尔帕莱索天主教大学制作和主办Elsevier B. V.保留所有权利。
Background: In the present study populations, representing different rounds of recombination were used for the analysis of phenotypic effects associated with the sdw1/denso locus. Other studies have mostly focused only on one type of population. Many different QTLs mapped at the same position as the sdw1/denso locus may indicate a pleiotropy of this gene or a tight linkage between genes conditioning quantitative traits. To date, results of studies have not unequivocally proven either of these two phenomena.Results: Both breeding and molecular mapping experiments were undertaken to examine 200 single seed descent (SSD) and 60 doubled haploid (DH) lines obtained from the Maresi (with a semi-dwarfing gene) and Pomo cross combination. They were evaluated for the type of juvenile growth habit and certain agronomic traits were measured after harvesting. The estimates of mean values, standard errors and significance of effects were analyzed. In terms of the analyzed characteristics, the greatest variability was obtained for genotypes with the prostrate growth habit. Microsatellite markers (SSR) were also used to identify co-segregation with the sdw1/denso locus and Bmag0013, Bmag0877, Bmag0306b markers were linked the closest. A partial linkage map of chromosome 3H with the sdw1/denso semi-dwarfing gene was constructed and QTLs were identified.Conclusions: Our experiments confirmed the impact of the semi-dwarfing gene on plant height, heading and flowering date both in SSD and DH populations, which may indicate pleiotropy. Moreover, a partial linkage between sdw1/denso locus and grain weight per spike and 1000-grain weight was found in the SSD population. (C) 2014 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.