INVERTED REPEATS IN CHLOROPLAST DNA FROM HIGHER-PLANTS
INVERTED REPEATS IN CHLOROPLAST DNA FROM HIGHER-PLANTS
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DOI：
10.1073/pnas.76.1.41
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
TEWARI, KK
中科院分区：
文献类型：
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作者：
KOLODNER, R;TEWARI, KK
来自菠菜（Spinacia oleracea）、生菜（Lactuca sativa）和玉米（Zea mays）植物的环状叶绿体DNA通过电子显微镜进行了检测，发现含有一个大的反向重复1次的序列。菠菜和莴苣叶绿体DNA的反向序列长达24,400个碱基对。玉米叶绿体DNA的反向序列长达22500个碱基对。变性图谱研究表明，倒排序列的结构在这3种植物中高度保守。豌豆（Pisum sativum）叶绿体DNA不包含反向重复序列。豌豆叶绿体DNA的所有环状二聚体都是头对尾的构象。菠菜和生菜的圆形二聚体也具有头到尾的构象。然而，在生菜和菠菜叶绿体DNA的制备中，大约70-80%的环状二聚体是头对头的构象。头对头的圆形二聚体可能是由两个圆形单体倒序的重组事件形成的。
The circular chloroplast DNA from spinach [Spinacia oleracea], lettuce [Lactuca sativa] and corn [Zea mays] plants were examined by EM and shown to contain a large sequence repeated 1 time in reverse polarity. The inverted sequence in spinach and lettuce chloroplast DNA is 24,400 base pairs long. The inverted sequence in the corn chloroplast DNA is 22,500 base pairs long. Denaturation mapping studies showed that the structure of the inverted sequence is highly conserved in these 3 plants. Pea [Pisum sativum] chloroplast DNA does not contain an inverted repeat. All of the circular dimers of pea chloroplast DNA are in a head-to-tail conformation. Circular dimers of spinach and lettuce also had head-to-tail conformation. However, approximately 70-80% of the circular dimers in preparations of lettuce and spinach chloroplast DNA were in a head-to-head conformation. The head-to-head circular dimers probably are formed by a recombination event between 2 circular monomers in the inverted sequence.