A new approach for improving epothilone B yield in Sorangium cellulosum by the introduction of vgb epoF genes
A new approach for improving epothilone B yield in Sorangium cellulosum by the introduction of vgb epoF genes
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通过引入 vgb epoF 基因提高 Sorangium cellulosum 埃坡霉素 B 产量的新方法
DOI：
10.1007/s10295-016-1735-9
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发表时间：
2016
影响因子：
3.4
通讯作者：
Liu Taomei
中科院分区：
文献类型：
--
作者：
Ye Wei;Zhang Weimin;Chen Yuchan;Li Haohua;Li Saini;Pan Qingling;Tan Guohui;Liu Taomei
与紫杉醇相比，Epothilone B具有更强的抗癌活性和更弱的副作用，因此引起了人们的极大关注。Epothilone B的产率较低，限制了其应用。在本研究中，我们报道了首次通过电穿孔将vgb基因和epF基因导入到纤维索兰氏菌M4中，并通过Southern印迹分析证实了这一点。QRT-PCR、SDS-PAGE和免疫印迹分析结果证实了vgbandepoF基因的转录和表达。LC-MS结果表明，环磷酰胺类化合物B、A的产率有所提高，环磷酰胺类化合物D、C的产率有所下降。将vgb基因、EpoF基因和两种基因导入菌株So ce M4后，产量分别提高了57.9%±0.03%、62.7%±0.08%和122.4%±0.7%。本研究为提高Epothilone B的产率提供了一条新的途径。纤维素酶。
Epothilone B has drawn great attention due to its much stronger anticancer activity and weaker side effects compared with taxol. The relative low yield of epothilone B limited its application. In this study, we report the successful introduction of thevgbgene and theepoFgene intoSorangium cellulosumSo ce M4 by electroporation for the first time, which was demonstrated by Southern blot analysis. Results of qRT-PCR, SDS-PAGE and western blot analysis confirmed the transcription and expression of thevgbandepoFgenes. LC–MS results showed that the epothilones B, A yields were improved and epothilones D, C yields were decreased. The yields of epothilone B were improved by 57.9 ± 0.3, 62.7 ± 0.8 and 122.4 ± 0.7 % through the introduction ofvgbgene,epoFgene and both genes into strain So ce M4, respectively. Our study provides a new approach for improving epothilone B yield inS. cellulosum.