CDNA CLONING OF ESTERASE-1, THE MAJOR ESTERASE-ACTIVITY IN MOUSE PLASMA
CDNA CLONING OF ESTERASE-1, THE MAJOR ESTERASE-ACTIVITY IN MOUSE PLASMA
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DOI：
10.1016/s0006-291x(88)80513-8
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发表时间：
1988-03-30
影响因子：
3.1
通讯作者：
FINLAY, TH
中科院分区：
文献类型：
--
作者：
GENETTA, TL;DEUSTACHIO, P;FINLAY, TH
我们在这里报告的酯酶1，在小鼠血浆中的主要酯酶活性的部分cDNA的克隆。从小鼠肝poly A+ RNA构建的λ gt 11 cDNA文库中分离到470个碱基对的插入片段，并通过杂交选择翻译鉴定。我们表明，性二态性显示在该蛋白质的血浆水平是由转录水平的差异。此外，使用小鼠重组近交系的RFLP数据映射在小鼠8号染色体上的Es-1位点的克隆。
We report here the cloning of a partial cDNA for Esterase 1, the major esterase activity in mouse plasma. A 470 base pair insert was isolated from a lambda gt11 cDNA library constructed from mouse liver poly A+ RNA, and identified by hybrid selected translation. We show that the sexual dimorphism displayed in the plasm levels of this protein is caused by a difference at the level of transcription. In addition, RFLP data using mouse recombinant inbred strains mapped this clone at the Es-1 locus on mouse chromosome 8.