H-NS Regulates Gene Expression and Compacts the Nucleoid: Insights from Single-Molecule Experiments.
H-NS Regulates Gene Expression and Compacts the Nucleoid: Insights from Single-Molecule Experiments.
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DOI：
10.1016/j.bpj.2015.08.016
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发表时间：
2015-10
影响因子：
3.4
通讯作者：
Ricksen S. Winardhi;Jie Yan;L. Kenney
中科院分区：
文献类型：
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作者：
Ricksen S. Winardhi;Jie Yan;L. Kenney
一组丰富的类核相关蛋白(NAP)在组织细菌染色体和调节基因转录方面发挥着关键作用。组蛋白样核结构蛋白(H-NS)是广泛存在于细菌物种中的NAP家族的代表。由于它们在细菌病原体的基因沉默中的关键作用而引起了广泛的关注。近年来，单分子操作和成像技术的快速发展使得直接探测H-NS与DNA的结合、它对DNA构象和拓扑的影响以及它与其他DNA结合蛋白在单DNA分子水平上的竞争成为可能。在这里，我们回顾了这些研究的最新发现，并就这些发现如何为理解H-NS家族成员在DNA组织和基因沉默中的作用提供了我们的观点。
A set of abundant nucleoid-associated proteins (NAPs) play key functions in organizing the bacterial chromosome and regulating gene transcription globally. Histone-like nucleoid structuring protein (H-NS) is representative of a family of NAPs that are widespread across bacterial species. They have drawn extensive attention due to their crucial function in gene silencing in bacterial pathogens. Recent rapid progress in single-molecule manipulation and imaging technologies has made it possible to directly probe DNA binding by H-NS, its impact on DNA conformation and topology, and its competition with other DNA-binding proteins at the single-DNA-molecule level. Here, we review recent findings from such studies, and provide our views on how these findings yield new insights into the understanding of the roles of H-NS family members in DNA organization and gene silencing.